B8GBG7 (SYN_CHLAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Cagg_1900

Organism

Chloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP]

Taxonomic identifier

326427 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000146481

Sequences

	Sequence		Length	Mass (Da)
	B8GBG7 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 744DE1F1EBB3E3AC	FASTA	440	49,957
	10 20 30 40 50 60 MSLLPTATVA TIARHVGQRV TLAGWVYHKT EKGKLIFILL RDGSGTIQCV TFKKNVSEAT 70 80 90 100 110 120 FATAQALTQE SSCRITGSVR ADERAPGGYE LDVEEIELVG PSHEYPITPK EHGVEFLMEH 130 140 150 160 170 180 RHLWVRSAKQ HAILRIRAEV IAAAQEWLNE QGFVRFDTPI LTATAAEGTT NLFATDYFDL 190 200 210 220 230 240 GKAYLAQTGQ LYVEAGMMSF GKVYCFGPTF RAEKSKTRRH LTEFWMIEPE VAFADHEDNM 250 260 270 280 290 300 RLQEQFVSAI VARVLDRRRE DLKVLERDIS LLEQVVPPFP RITYDQAIEL IAAHQGEVEG 310 320 330 340 350 360 AYPLPWGEDF GAPHETLIAS KFDRPVFVER FPSAVKAFYM QPDPNRPEVA LCADLLAPEG 370 380 390 400 410 420 YGEIIGGSQR IHDPDLLEQR IRAHGLRLED YQWYLDLRRY GTVPHSGFGM GIERVVAWIT 430 440 GTRHIRETIP FPRQLYRIYP

References

[1]

"Complete sequence of Chloroflexus aggregans DSM 9485." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001337 Genomic DNA. Translation: ACL24795.1.
RefSeq	YP_002463231.1. NC_011831.1.
3D structure databases
ProteinModelPortal	B8GBG7.
ModBase	Search...
Protein-protein interaction databases
STRING	B8GBG7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7266391.
GenomeReviews	Gene locus Cagg_1900 in contig CP001337_GR.
KEGG	cag:Cagg_1900.
PATRIC	21406369. VBIChlAgg85409_1971.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	DHLPQET.
ProtClustDB	PRK03932.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CHLAD

Accession

Primary (citable) accession number: B8GBG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program