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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Chloroflexus aggregans (strain MD-66 / DSM 9485)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571S-adenosyl-L-methionineUniRule annotation
Binding sitei82 – 821S-adenosyl-L-methionineUniRule annotation
Binding sitei109 – 1091S-adenosyl-L-methionineUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCAGG326427:GHS8-3798-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Cagg_3746
OrganismiChloroflexus aggregans (strain MD-66 / DSM 9485)
Taxonomic identifieri326427 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
ProteomesiUP000002508 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224tRNA (guanine-N(7)-)-methyltransferasePRO_0000387943Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi326427.Cagg_3746.

Structurei

3D structure databases

ProteinModelPortaliB8GAY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000033410.
KOiK03439.
OMAiMISVISD.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8GAY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRGRYPARL KVTVPPPEIA ARYYRSWPAH ELYHHPDRFP LLNAESLFGR
60 70 80 90 100
NAPLTLELGC ATGEYICTLA TAQPDACFVG VDIVAKPLYR AVERAVELGL
110 120 130 140 150
ENIIFLQADA RLLYQRIPDC VLTTIILHFP PPLLRNRQRN QLLVSAQMLG
160 170 180 190 200
CAERSLVPGG YLSFLTDHPD LFSLMQELLP AFPRLRGLPA SPSELAVFES
210 220
HYHRRWAARG REIRGLRIER IAEE
Length:224
Mass (Da):25,377
Last modified:March 3, 2009 - v1
Checksum:i2E00EAA7073FFEB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001337 Genomic DNA. Translation: ACL26582.1.
RefSeqiWP_015942427.1. NC_011831.1.
YP_002465018.1. NC_011831.1.

Genome annotation databases

EnsemblBacteriaiACL26582; ACL26582; Cagg_3746.
KEGGicag:Cagg_3746.
PATRICi21410214. VBIChlAgg85409_3866.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001337 Genomic DNA. Translation: ACL26582.1.
RefSeqiWP_015942427.1. NC_011831.1.
YP_002465018.1. NC_011831.1.

3D structure databases

ProteinModelPortaliB8GAY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326427.Cagg_3746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL26582; ACL26582; Cagg_3746.
KEGGicag:Cagg_3746.
PATRICi21410214. VBIChlAgg85409_3866.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000033410.
KOiK03439.
OMAiMISVISD.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciCAGG326427:GHS8-3798-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Chloroflexus aggregans DSM 9485."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MD-66 / DSM 9485.

Entry informationi

Entry nameiTRMB_CHLAD
AccessioniPrimary (citable) accession number: B8GAY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: March 3, 2009
Last modified: April 29, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.