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B8GAY2 (TRMB_CHLAD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Cagg_3746
OrganismChloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP]
Taxonomic identifier326427 [NCBI]
Taxonomic lineageBacteriaChloroflexiChloroflexalesChloroflexaceaeChloroflexus

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000387943

Sites

Binding site571S-adenosyl-L-methionine By similarity
Binding site821S-adenosyl-L-methionine By similarity
Binding site1091S-adenosyl-L-methionine By similarity
Binding site1671Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B8GAY2 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 2E00EAA7073FFEB0

FASTA22425,377
        10         20         30         40         50         60 
MGRGRYPARL KVTVPPPEIA ARYYRSWPAH ELYHHPDRFP LLNAESLFGR NAPLTLELGC 

        70         80         90        100        110        120 
ATGEYICTLA TAQPDACFVG VDIVAKPLYR AVERAVELGL ENIIFLQADA RLLYQRIPDC 

       130        140        150        160        170        180 
VLTTIILHFP PPLLRNRQRN QLLVSAQMLG CAERSLVPGG YLSFLTDHPD LFSLMQELLP 

       190        200        210        220 
AFPRLRGLPA SPSELAVFES HYHRRWAARG REIRGLRIER IAEE 

« Hide

References

[1]"Complete sequence of Chloroflexus aggregans DSM 9485."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001337 Genomic DNA. Translation: ACL26582.1.
RefSeqYP_002465018.1. NC_011831.1.

3D structure databases

ProteinModelPortalB8GAY2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326427.Cagg_3746.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL26582; ACL26582; Cagg_3746.
GeneID7267819.
KEGGcag:Cagg_3746.
PATRIC21410214. VBIChlAgg85409_3866.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000033410.
KOK03439.
OMAELYHHPD.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK955837.

Enzyme and pathway databases

BioCycCAGG326427:GHS8-3798-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CHLAD
AccessionPrimary (citable) accession number: B8GAY2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways