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B8GAA4

- PFKA_CHLAD

UniProt

B8GAA4 - PFKA_CHLAD

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Chloroflexus aggregans (strain MD-66 / DSM 9485)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi116 – 1161Magnesium; catalyticUniRule annotation
Sitei117 – 1171Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei140 – 1401Proton acceptorUniRule annotation
Binding sitei175 – 1751Substrate; shared with dimeric partnerUniRule annotation
Binding sitei235 – 2351SubstrateUniRule annotation
Binding sitei272 – 2721Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 792ATPUniRule annotation
Nucleotide bindingi115 – 1184ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCAGG326427:GHS8-3694-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Cagg_3643
OrganismiChloroflexus aggregans (strain MD-66 / DSM 9485)
Taxonomic identifieri326427 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
ProteomesiUP000002508: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356ATP-dependent 6-phosphofructokinasePRO_1000192367Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi326427.Cagg_3643.

Structurei

3D structure databases

ProteinModelPortaliB8GAA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni138 – 1403Substrate bindingUniRule annotation
Regioni182 – 1843Substrate bindingUniRule annotation
Regioni278 – 2814Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiNEICKAM.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8GAA4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASKKQRIGV LTSGGDAPGL NAVIRAVVKS ASSLGWEVIG IHDGFEGLLG
60 70 80 90 100
TKSYRVLTNA DVQGLLPRGG TILRTTNKGH FGPRRSDELS EADPYVRAVK
110 120 130 140 150
AIEEMGLRAL ITIGGEGTQR IALELHKLGA PVIGVPKTID NDLAGTDRTF
160 170 180 190 200
GFDTALQVAT DAIDRLHTTA ASHNRVMVLE VMGRHTGWIA LHAGLAGGAD
210 220 230 240 250
VILIPEIPFT IERVAEKVHA RDAQGSAFSI IVVAEGARPR GGSEMYIAEG
260 270 280 290 300
RLGGIGHWVG EQLERLTGKD VRVVVLGHLQ RGGSPSPYDR LLSTRYGAAA
310 320 330 340 350
VQAAARGIYG EMVALRGQDI VTVPLAEACG YLNRVRPHSD LVLCARSLGI

VFGDEL
Length:356
Mass (Da):37,734
Last modified:March 3, 2009 - v1
Checksum:i66436D7A5E6F4579
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001337 Genomic DNA. Translation: ACL26479.1.
RefSeqiWP_015942324.1. NC_011831.1.
YP_002464915.1. NC_011831.1.

Genome annotation databases

EnsemblBacteriaiACL26479; ACL26479; Cagg_3643.
GeneIDi7269787.
KEGGicag:Cagg_3643.
PATRICi21409998. VBIChlAgg85409_3759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001337 Genomic DNA. Translation: ACL26479.1 .
RefSeqi WP_015942324.1. NC_011831.1.
YP_002464915.1. NC_011831.1.

3D structure databases

ProteinModelPortali B8GAA4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326427.Cagg_3643.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL26479 ; ACL26479 ; Cagg_3643 .
GeneIDi 7269787.
KEGGi cag:Cagg_3643.
PATRICi 21409998. VBIChlAgg85409_3759.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi NEICKAM.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci CAGG326427:GHS8-3694-MONOMER.

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Chloroflexus aggregans DSM 9485."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MD-66 / DSM 9485.

Entry informationi

Entry nameiPFKA_CHLAD
AccessioniPrimary (citable) accession number: B8GAA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3