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B8GAA4

- PFKA_CHLAD

UniProt

B8GAA4 - PFKA_CHLAD

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, Cagg_3643
Organism
Chloroflexus aggregans (strain MD-66 / DSM 9485)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogen By similarity
Metal bindingi116 – 1161Magnesium; catalytic By similarity
Sitei117 – 1171Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity
Active sitei140 – 1401Proton acceptor By similarity
Binding sitei175 – 1751Substrate; shared with dimeric partner By similarity
Binding sitei235 – 2351Substrate By similarity
Binding sitei272 – 2721Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 792ATP By similarity
Nucleotide bindingi115 – 1184ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCAGG326427:GHS8-3694-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:Cagg_3643
OrganismiChloroflexus aggregans (strain MD-66 / DSM 9485)
Taxonomic identifieri326427 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
ProteomesiUP000002508: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_1000192367Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi326427.Cagg_3643.

Structurei

3D structure databases

ProteinModelPortaliB8GAA4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni138 – 1403Substrate binding By similarity
Regioni182 – 1843Substrate binding By similarity
Regioni278 – 2814Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiNEICKAM.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8GAA4-1 [UniParc]FASTAAdd to Basket

« Hide

MASKKQRIGV LTSGGDAPGL NAVIRAVVKS ASSLGWEVIG IHDGFEGLLG    50
TKSYRVLTNA DVQGLLPRGG TILRTTNKGH FGPRRSDELS EADPYVRAVK 100
AIEEMGLRAL ITIGGEGTQR IALELHKLGA PVIGVPKTID NDLAGTDRTF 150
GFDTALQVAT DAIDRLHTTA ASHNRVMVLE VMGRHTGWIA LHAGLAGGAD 200
VILIPEIPFT IERVAEKVHA RDAQGSAFSI IVVAEGARPR GGSEMYIAEG 250
RLGGIGHWVG EQLERLTGKD VRVVVLGHLQ RGGSPSPYDR LLSTRYGAAA 300
VQAAARGIYG EMVALRGQDI VTVPLAEACG YLNRVRPHSD LVLCARSLGI 350
VFGDEL 356
Length:356
Mass (Da):37,734
Last modified:March 3, 2009 - v1
Checksum:i66436D7A5E6F4579
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001337 Genomic DNA. Translation: ACL26479.1.
RefSeqiYP_002464915.1. NC_011831.1.

Genome annotation databases

EnsemblBacteriaiACL26479; ACL26479; Cagg_3643.
GeneIDi7269787.
KEGGicag:Cagg_3643.
PATRICi21409998. VBIChlAgg85409_3759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001337 Genomic DNA. Translation: ACL26479.1 .
RefSeqi YP_002464915.1. NC_011831.1.

3D structure databases

ProteinModelPortali B8GAA4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326427.Cagg_3643.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL26479 ; ACL26479 ; Cagg_3643 .
GeneIDi 7269787.
KEGGi cag:Cagg_3643.
PATRICi 21409998. VBIChlAgg85409_3759.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi NEICKAM.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci CAGG326427:GHS8-3694-MONOMER.

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Chloroflexus aggregans DSM 9485."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MD-66 / DSM 9485.

Entry informationi

Entry nameiPFKA_CHLAD
AccessioniPrimary (citable) accession number: B8GAA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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