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B8G933 (GLYA_CHLAD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:Cagg_3468
OrganismChloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP]
Taxonomic identifier326427 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_1000195436

Regions

Region122 – 1243Substrate binding By similarity

Sites

Binding site321Pyridoxal phosphate By similarity
Binding site521Pyridoxal phosphate By similarity
Binding site541Substrate By similarity
Binding site611Substrate binding By similarity
Binding site621Pyridoxal phosphate By similarity
Binding site1181Substrate By similarity
Binding site1731Pyridoxal phosphate By similarity
Binding site2011Pyridoxal phosphate By similarity
Binding site2261Pyridoxal phosphate By similarity
Binding site2331Pyridoxal phosphate By similarity
Binding site2581Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3581Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2271N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
B8G933 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 54E46B79E9C2C13E

FASTA41845,121
        10         20         30         40         50         60 
MLEHLRATDP IIADLIEREA QRQRQGLELI ASENYTSLAV MEAQGSVLTN KYAEGLPGRR 

        70         80         90        100        110        120 
YYGGCEFVDA IEQLAIDRAC QLFGTSHANV QPHSGAQANI AVFTALLQPG DTILGMRLDH 

       130        140        150        160        170        180 
GGHLTHGSPV NFSGKWYNVH FYGVDPQTGQ IDYDDLAAKA RAIRPKLITS GASAYPRLID 

       190        200        210        220        230        240 
FARMRQIADE VGALLMADIA HIAGLVATGE HPSPVGHAHI ITTTTHKTLR GPRGGLILMG 

       250        260        270        280        290        300 
EEFAKQINSS VFPGTQGGPL MHVIAGKAVA FGEALRPEFK QYAAQIRRNA KALAEGLHAQ 

       310        320        330        340        350        360 
GLTLVSGGTD NHLMLVDLRS TGLTGAQAQR ALDKAAITVN KNAIPDDPQP PMKTSGIRIG 

       370        380        390        400        410 
TPAVTTRGMR EREMAQIAAW IGEVLMYPDD EVRLARIAAE VAEMCRHFPV PADMVQVR

« Hide

References

[1]"Complete sequence of Chloroflexus aggregans DSM 9485."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001337 Genomic DNA. Translation: ACL26308.1.
RefSeqYP_002464744.1. NC_011831.1.

3D structure databases

ProteinModelPortalB8G933.
SMRB8G933. Positions 2-404.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8G933.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7269693.
GenomeReviewsGene locus Cagg_3468 in contig CP001337_GR.
KEGGcag:Cagg_3468.
PATRIC21409628. VBIChlAgg85409_3576.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG301263.
OMAIFNAGYA.
ProtClustDBPRK00011.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_CHLAD
AccessionPrimary (citable) accession number: B8G933
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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