B8G8J2 (B8G8J2_CHLAD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ACL24254.1
EC=5.99.1.2 EMBL ACL24254.1

Gene names

Ordered Locus Names:

Cagg_1347

Organism

Chloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP] EMBL ACL24254.1

Taxonomic identifier

326427 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS005733

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B8G8J2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: A0E1F25BD41CE517	FASTA	780	87,434
	10 20 30 40 50 60 MGEKVVIVES PAKARTIQKY LGKGYKVTSS MGHVRDLPKN GLAIDIEHDF APSYEIVKPK 70 80 90 100 110 120 VVSELNQAVR NADAIYLATD PDREGEAIAW HITQAVKTPK KTPIYRVVFQ EITRNAVQQA 130 140 150 160 170 180 LQQPRQINQN LVDAQQARRV LDRLVGYQLS PLLWDKVKRG LSAGRVQSVA VRLIVERERE 190 200 210 220 230 240 IENFKPQEYW TIEADLLKEA GIAPRDLFRA TLIERDGKKL EKFSIERREQ AEAIVADLQG 250 260 270 280 290 300 AAYTVLKVTR RDKRRSPPPP FTTSTLQQEA ARKLGFSAKK TMMLAQRLYE GVDIGGEEGM 310 320 330 340 350 360 VGLITYMRTD SVQVAAEAQA EAREVIDRRF GREYLPDQPP VYKIKAKGAQ EAHEAIRPTS 370 380 390 400 410 420 SARTPEQLSE RLERDLWRLY DLIWKRFIAS QMAPAIFDST TVDIAAQPSV AGAPPYLFRA 430 440 450 460 470 480 TGSVLKFPGF LAVYNVSLDE GEEDEDSERR LPPLVEGENL QLVELLPVQH FTEPPPRYTE 490 500 510 520 530 540 ASLVKELERL GIGRPSTYAT ILSTIQEREY VEMVDKKLIP TMLGRIVTDL LVEHFGNIVD 550 560 570 580 590 600 YDFTSSLEQQ LDDIAEGSKQ WVPVLREFYG PFRSTLETAQ RQMRNVKREE IITDLDCPKC 610 620 630 640 650 660 GKGKLVIKFG RNGEFLACSR YNREGEGDSC DFTGDFHRDE NGNIVLDQAS APETSDVLCN 670 680 690 700 710 720 VCGRPMVIKK SRFGPFLGCS GYPECTNTRR IGRDGKPVPL PEPTGVTCPK CGEGELLRRR 730 740 750 760 770 780 GKFGRPFYGC SRYPKCDYIT NSLDEAQAGV AVEAAPALPP TVEKPAAPAR KSSGKTRKSA

References

[1]

"Complete sequence of Chloroflexus aggregans DSM 9485." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001337 Genomic DNA. Translation: ACL24254.1.
RefSeq	YP_002462690.1. NC_011831.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8G8J2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7268639.
GenomeReviews	Gene locus Cagg_1347 in contig CP001337_GR.
KEGG	cag:Cagg_1347.
PATRIC	21405206. VBIChlAgg85409_1396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8G8J2_CHLAD

Accession

Primary (citable) accession number: B8G8J2

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)