B8G6Y1 (KITH_CHLAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	Cagg_3082

Organism

Chloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP]

Taxonomic identifier

326427 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus › Chloroflexus aggregans

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Thymidine kinase HAMAP-Rule MF_00124		PRO_1000122653
Regions
	Nucleotide binding	15 – 22	8	ATP By similarity
	Nucleotide binding	87 – 90	4	ATP By similarity
Sites
	Active site	88	1	Proton acceptor Potential
	Metal binding	147	1	Zinc By similarity
	Metal binding	150	1	Zinc By similarity
	Metal binding	185	1	Zinc By similarity
	Metal binding	188	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8G6Y1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: D41D758DFFB0B930	FASTA	193	21,414
	10 20 30 40 50 60 MTRPSEGGRI EVICGCMYSG KTEELIRRMR QVRIARQSYR IFTPRMDTRY AEGHVASHSG 70 80 90 100 110 120 SRLEAITVAT MCEILAYARD VQVVAIDELH LFDDDPAEMV RGCQVLADRG ARVIVAGLDL 130 140 150 160 170 180 NYRAEPFPAM MHLLALAEQV DKLYAICVKC GAYATRSQRL IDGKPAPANA PTIVVGGLDM 190 YEARCRACYE PAL

References

[1]

"Complete sequence of Chloroflexus aggregans DSM 9485." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001337 Genomic DNA. Translation: ACL25940.1.
RefSeq	YP_002464376.1. NC_011831.1.
3D structure databases
ProteinModelPortal	B8G6Y1.
ModBase	Search...
Protein-protein interaction databases
STRING	326427.Cagg_3082.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL25940; ACL25940; Cagg_3082.
GeneID	7269499.
KEGG	cag:Cagg_3082.
PATRIC	21408848. VBIChlAgg85409_3191.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076390.
KO	K00857.
OMA	KVHAICV.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	CAGG326427:GHS8-3123-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_CHLAD

Accession

Primary (citable) accession number: B8G6Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program