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B8G6P6 (TRUA_CHLAD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene names
Name:truA
Ordered Locus Names:Cagg_2995
OrganismChloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP]
Taxonomic identifier326427 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus ›

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278tRNA pseudouridine synthase A HAMAP-Rule MF_00171
PRO_1000194538

Sites

Active site521Nucleophile By similarity
Binding site1101Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B8G6P6 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: D7B1ED706EEED7A7

FASTA27830,997
        10         20         30         40         50         60 
MRTIALLLAY DGTDFAGSQW QTDIRTVQGA IEAAWEALTQ EQRRIVLAGR TDAGVHASGQ 

        70         80         90        100        110        120 
VAHVQTATRH SLATIWRGLN AHLPTDVTVQ NVGEAVRDFH ARYSAIEREY RYLIDCAPVP 

       130        140        150        160        170        180 
LPQLRHQALH YAGTLDVTAM ATALKLLEGT HDFAAFTTAT PSQRSTVRTM YWTRIVERVW 

       190        200        210        220        230        240 
FDRRLLAIEL AANAFLQHMV RMIVGTLLLV GRGRMTVDQF GEVLAGRDRR LAGPTAPAHG 

       250        260        270 
LTLTAVRYPP GLIRWVEPLE RQQGATKVEQ PSSYVHEE

« Hide

References

[1]"Complete sequence of Chloroflexus aggregans DSM 9485."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001337 Genomic DNA. Translation: ACL25855.1.
RefSeqYP_002464291.1. NC_011831.1.

3D structure databases

ProteinModelPortalB8G6P6.
ModBaseSearch...

Protein-protein interaction databases

STRING326427.Cagg_2995.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL25855; ACL25855; Cagg_2995.
GeneID7266526.
KEGGcag:Cagg_2995.
PATRIC21408672. VBIChlAgg85409_3103.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000248674.
KOK06173.
OMAGKSFLWE.
ProtClustDBPRK14588.

Enzyme and pathway databases

BioCycCAGG326427:GHS8-3036-MONOMER.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMSSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUA_CHLAD
AccessionPrimary (citable) accession number: B8G6P6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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