B8G6F7 (SYM_CHLAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Cagg_0976

Organism

Chloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP]

Taxonomic identifier

326427 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus › Chloroflexus aggregans

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Methionine--tRNA ligase HAMAP-Rule MF_00098		PRO_1000118728
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP-Rule MF_00098
	Motif	351 – 355	5	"KMSKS" region HAMAP-Rule MF_00098
Sites
	Metal binding	144	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	157	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity
	Binding site	354	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8G6F7 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: DCFE8361F05F6ADC	FASTA	600	68,054
	10 20 30 40 50 60 MPEHILVAVA WPYANGPRHI GHVAGFGVPA DIFARYHRLR GNRVLMVSGT DEHGTPITLV 70 80 90 100 110 120 ADKEGTTPQA IADRYNKIIG DDLYNLGLSY DTFTRTTTAN HYAVTQDIFR TLYERGYIIR 130 140 150 160 170 180 QETLGAFSAT TGRTLPDRYI EGTCPLCGYD EARGDQCDNC GSQLDPTDLI NPRSKVDGQP 190 200 210 220 230 240 PVFKPTEHFF LDLPAFAEQL HDWINRQDHW RPNVRNFSLN FLKDLKPRAI TRDLEWGVPI 250 260 270 280 290 300 PLPEYANRDD KKIYVWFDAV IGYLSASIEW AQNSGQPDAW REWWQNPDAR HFYFMGKDNI 310 320 330 340 350 360 VFHTVIWPAM LLGYGAGGQF GADPGGKYDG IPLQLPYNVV SSEFLTMEGK KFSSSRGIVI 370 380 390 400 410 420 YVNDFLSRYD ADALRYFLTI AGPENQDTDF TWAEFVRRNN DELVATWGNL VNRTLSNVYK 430 440 450 460 470 480 NFGSVPQPGP LTPVDEQVLA EVTGGIETVG ELLAAARFKA ALAEAMRLAA QVNIYLSEQE 490 500 510 520 530 540 PWKVIKSDHE RAATIWYVAL RCVDTLKIIF TPFLPFSSQR LHEYLGYNGY IAGPLTFREV 550 560 570 580 590 600 TEANGRTHRV LTGDYDRWVG RWEPSVLPVG QVLRQPQPLF KKLDEKVIEE ELARMQSRLG

References

[1]

"Complete sequence of Chloroflexus aggregans DSM 9485." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001337 Genomic DNA. Translation: ACL23894.1.
RefSeq	YP_002462330.1. NC_011831.1.
3D structure databases
ProteinModelPortal	B8G6F7.
ModBase	Search...
Protein-protein interaction databases
STRING	326427.Cagg_0976.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL23894; ACL23894; Cagg_0976.
GeneID	7268348.
KEGG	cag:Cagg_0976.
PATRIC	21404432. VBIChlAgg85409_1015.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200402.
KO	K01874.
OMA	SYYFMGK.
ProtClustDB	CLSK2477367.
Enzyme and pathway databases
BioCyc	CAGG326427:GHS8-986-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_CHLAD

Accession

Primary (citable) accession number: B8G6F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program