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B8G5R5 (DEF_CHLAD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide deformylase

Short name=PDF
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase
Gene names
Name:def
Ordered Locus Names:Cagg_0857
OrganismChloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP]
Taxonomic identifier326427 [NCBI]
Taxonomic lineageBacteriaChloroflexiChloroflexalesChloroflexaceaeChloroflexus

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP-Rule MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP-Rule MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP-Rule MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Peptide deformylase HAMAP-Rule MF_00163
PRO_1000200720

Sites

Active site1531 By similarity
Metal binding1091Iron By similarity
Metal binding1521Iron By similarity
Metal binding1561Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
B8G5R5 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 5EACAD0B7149E601

FASTA18821,429
        10         20         30         40         50         60 
MAIRRILRID DAEDRKILKM QCRPVKLPDR NLKQLVADMF ETMHAANGVG LAAPQIGIPI 

        70         80         90        100        110        120 
QLCIIEIPPE YEEQPDGSLI EVNPAEPYVL INPRIVKTSG EEIMRDEGCL SLPGWYGMVP 

       130        140        150        160        170        180 
RQTWVTVEFQ DLSGKHHRLR RADGLLGWAI QHEVDHLHGI LFTERIRDLS TLRDITKERE 


AQPVEAKA 

« Hide

References

[1]"Complete sequence of Chloroflexus aggregans DSM 9485."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001337 Genomic DNA. Translation: ACL23776.1.
RefSeqYP_002462212.1. NC_011831.1.

3D structure databases

ProteinModelPortalB8G5R5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326427.Cagg_0857.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL23776; ACL23776; Cagg_0857.
GeneID7268308.
KEGGcag:Cagg_0857.
PATRIC21404182. VBIChlAgg85409_0894.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0242.
HOGENOMHOG000243508.
KOK01462.
OMALETMYQA.
OrthoDBEOG664CMF.
ProtClustDBCLSK973314.

Enzyme and pathway databases

BioCycCAGG326427:GHS8-863-MONOMER.

Family and domain databases

Gene3D3.90.45.10. 1 hit.
HAMAPMF_00163. Pep_deformylase.
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERPTHR10458. PTHR10458. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. SSF56420. 1 hit.
TIGRFAMsTIGR00079. pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF_CHLAD
AccessionPrimary (citable) accession number: B8G5R5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families