Skip Header

Contribute Send feedback
Read comments (?) or add your own

B8G509 (B8G509_CHLAD) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003
Ordered Locus Names:Cagg_0718
OrganismChloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP] EMBL ACL23642.1
Taxonomic identifier326427 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus

Protein attributes

Sequence length1066 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
B8G509 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 9282ADBB5439F42F

FASTA1,066120,273
        10         20         30         40         50         60 
MFKPVDPNVK FPQLEEEVLA WWDANDVVAK SLAAGEKPFV FYEGPPTANG RPGLHHTISR 

        70         80         90        100        110        120 
SFKDVILRYR SMQGYRIIGR REGWDTHGLP VEIEIEKKLG FSGKPDIERF GIAEFNRLCR 

       130        140        150        160        170        180 
ESVWEYIQEW KAFTKRIAFW LSEDAYITYE NDYIESTWWI FRQLWDRGLL FRDYKVTMHC 

       190        200        210        220        230        240 
PRCGTSLSDH EVSLGARDDV DDPSVYIKFR VKGTTLPPPA VDGTLEGAFL VAWTTTPWTL 

       250        260        270        280        290        300 
PANVALAVKH DAEYVEVEHN GERLVMAATL INQVLPAESF TVLRRFRGND LVGLRYEPLF 

       310        320        330        340        350        360 
RGVPGAGDTV DWETAYRVIA DEIVSLDDGT GIVHIAPAYG DLEVGRKHGL PTLFSVGLDG 

       370        380        390        400        410        420 
RVLPEFADLG FAGKFFKEAD PDITRNLKAR GLLLRSGRVR HYYPFCWRCG TPLLYYAKRS 

       430        440        450        460        470        480 
WYIRTTAFKA DLVANNQQIH WVPEHIRDGR FGNWLENNID WAISRERYWG TPIPIWTNAD 

       490        500        510        520        530        540 
GSHMVCIGSL AELEEKVGRS LRDLDLHRPY IDEVVWEDPD HGLMRRIPDV ADCWFDSGSM 

       550        560        570        580        590        600 
PVAQWHYPFE NRDVFEMSHP ADYICEAVDQ TRGWFYTLHA VSTLLFDRPA FKNVICLGHI 

       610        620        630        640        650        660 
LDKDGQKMSK SRGNVIEPQE VINAYGVDAL RWYLFTAAPP GNARRFSMDL VSESMRKFLL 

       670        680        690        700        710        720 
TLWNTYAFFV TYANLDRWQP NSGRTAELQP IDRWALAALN QLVQTATAAF EEYDVYSAAN 

       730        740        750        760        770        780 
AIEHFVDELS NWYVRRNRRR FWKSEGDADK EAAYQTLYTC LVTVAKLAAP FIPFVSEEIY 

       790        800        810        820        830        840 
RNLVAERDAS APESVHLARW PEVDQALLDD QLVADTEALL TAVSLGRAAR KQANIKVRQP 

       850        860        870        880        890        900 
LSELWLRAST PALLNGVRRF EAELRDELNV KVVRYLDANS AVVEYRLKPN LRLVGKKFGK 

       910        920        930        940        950        960 
LVPAITTALR DLTGDDARAA AQAVEAGQPV HLSVDGQTIE LLAEEVLVES SAPAGYAVAE 

       970        980        990       1000       1010       1020 
ADGMLVALNT TVTEELRLEG AARDLVRYVQ DARKSAGLAI SDRIRLFLSS TDEAALLAAT 

      1030       1040       1050       1060 
LAQHGAYIQN ETLAVELTVS APPAGAHVET DEFGDGEITI GVVKAG

« Hide

References

[1]"Complete sequence of Chloroflexus aggregans DSM 9485."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001337 Genomic DNA. Translation: ACL23642.1.
RefSeqYP_002462078.1. NC_011831.1.

3D structure databases

ProteinModelPortalB8G509.
SMRB8G509. Positions 1-835.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8G509.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7266970.
GenomeReviewsGene locus Cagg_0718 in contig CP001337_GR.
KEGGcag:Cagg_0718.
PATRIC21403892. VBIChlAgg85409_0749.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB8G509_CHLAD
AccessionPrimary (citable) accession number: B8G509
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info