B8G337 (B8G337_CHLAD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897

Gene names

Name:	gyrA HAMAP-Rule MF_01897
Ordered Locus Names:	Cagg_2331

Organism

Chloroflexus aggregans (strain MD-66 / DSM 9485) [Complete proteome] [HAMAP] EMBL ACL25210.1

Taxonomic identifier

326427 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Chloroflexales › Chloroflexaceae › Chloroflexus › Chloroflexus aggregans

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_01897
Ligand	ATP-binding SAAS SAAS013760 HAMAP-Rule MF_01897 DNA-binding SAAS SAAS005743 HAMAP-Rule MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase HAMAP-Rule MF_01897 SAAS SAAS005743
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	120	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

	Sequence		Length	Mass (Da)
	B8G337 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 4C21B4A2899D04A5	FASTA	824	91,669
	10 20 30 40 50 60 MEIGIVRPVS ITNEMRTAYL SYAMSVIVSR ALPDARDGLK PVQRRILYGM WDMGFRSNQP 70 80 90 100 110 120 YKKSARIVGD VLGKMHPHGD SAVYDALARM AQPWSMRYPL IDGQGNFGSI DGDPPAAMRY 130 140 150 160 170 180 TEARLAPIAE ELLSEIDKDT VDFRDNFDGS YREPVVLPAI LPNLLLNGAS GIAVGMATNI 190 200 210 220 230 240 PPHNLGELCD AISYLIDHPE ATVEELMEII PGPDFPTAGL ILGTEGILAA YSTGRGQITL 250 260 270 280 290 300 RAKTHIEEAG RGAYMIVVTE LPYQVNKARL QERIAELVKD RKIEGIRDVR DESDRNGMRL 310 320 330 340 350 360 VILLKQDAQP KKVLNALYKH TQMQTTFGIN MLALVEHGRQ PRVLTLKRLL QEYINHRQEV 370 380 390 400 410 420 IRRRTEFDLA RARERAHILE GLKIALDNLD AVIQTIRDSR TAESARNNLM RNFSLTERQA 430 440 450 460 470 480 QAILELQLRR LAALERQKIE EEYREVIKLI AELEDILANP RKVLHLIKED LSRLKEKYGD 490 500 510 520 530 540 PRRTRIIPDV TGEVSDEDLI PDVRVMITLT DRGYIKRQLP DAYRTQRRGG RGIKGMLTRE 550 560 570 580 590 600 QDIVRHLLTC NSLDNLLFFT DRGKVYQIKA HEVPDSSRTA KGLPLVNLVS LEPGEQVTSM 610 620 630 640 650 660 LAVREFDAEY LVMATVRGKI KRTQLREYSQ VRSNGLIAIG LEEGDVLGWV RVSHGHEDIL 670 680 690 700 710 720 LTTARGQTLR FEQSEVRPMG RPATGVIGIN LAEGDRVVSM DLAEEGADLL VVTARGIGKR 730 740 750 760 770 780 TALSEYPLKG RATGGVITIK LRNETDEVAA AAVVREHSLL TFITTGGMVM RTSASEISRL 790 800 810 820 GRATQGVTVV NVASGDRVAA LSVEDPDENG NGNGAAPTLI DPMG

References

[1]

"Complete sequence of Chloroflexus aggregans DSM 9485." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MD-66 / DSM 9485.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001337 Genomic DNA. Translation: ACL25210.1.
RefSeq	YP_002463646.1. NC_011831.1.
3D structure databases
ProteinModelPortal	B8G337.
SMR	B8G337. Positions 28-486.
ModBase	Search...
Protein-protein interaction databases
STRING	326427.Cagg_2331.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL25210; ACL25210; Cagg_2331.
GeneID	7268681.
KEGG	cag:Cagg_2331.
PATRIC	21407267. VBIChlAgg85409_2411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HOG000076279.
KO	K02469.
OMA	IAQEDVV.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	CAGG326427:GHS8-2362-MONOMER.
Family and domain databases
Gene3D	3.30.1360.40. 1 hit. 3.90.199.10. 2 hits.
HAMAP	MF_01897. GyrA.
InterPro	IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8G337_CHLAD

Accession

Primary (citable) accession number: B8G337

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)