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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Desulfitobacterium hafniense (strain DCB-2 / DSM 10664)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:ithiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441Substrate; via amide nitrogenUniRule annotation
Binding sitei120 – 1201ATPUniRule annotation
Binding sitei166 – 1661ATPUniRule annotation
Binding sitei193 – 1931Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDHAF272564:GCV8-2546-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:Dhaf_2519
OrganismiDesulfitobacterium hafniense (strain DCB-2 / DSM 10664)
Taxonomic identifieri272564 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfitobacterium
ProteomesiUP000007726 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Hydroxyethylthiazole kinasePRO_1000198116Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB8FUD3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiCMAGALV.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

B8FUD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKLTKALQ ALKHKTPLVH AITNVVTVND CANSLLAVGA SPAMCEAADE
60 70 80 90 100
VFEFSQLAGS LYLNLGTLTK EQEMAAYLAI RGATLKGIPV ILDPVACGAI
110 120 130 140 150
PRKKATIERL GHFGRFTVIK GNLGEVKALA GLAARVRGVD SLDEGNDGLE
160 170 180 190 200
ACQSLARAYG CVVAATGKVD IVADGQRACL IENGTEMLTR ITGAGCMAGA
210 220 230 240 250
LVAGFCGAYE DAFGATVAAL LTMSLAGELA QETSGGELPG TFRAHLIDQL
260
SLVDEALIEK RGRVQWL
Length:267
Mass (Da):27,844
Last modified:March 3, 2009 - v1
Checksum:i0322B0244338FD4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001336 Genomic DNA. Translation: ACL20547.1.
RefSeqiWP_005816651.1. NC_011830.1.

Genome annotation databases

EnsemblBacteriaiACL20547; ACL20547; Dhaf_2519.
KEGGidhd:Dhaf_2519.
PATRICi21662839. VBIDesHaf15223_2621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001336 Genomic DNA. Translation: ACL20547.1.
RefSeqiWP_005816651.1. NC_011830.1.

3D structure databases

ProteinModelPortaliB8FUD3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACL20547; ACL20547; Dhaf_2519.
KEGGidhd:Dhaf_2519.
PATRICi21662839. VBIDesHaf15223_2621.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiCMAGALV.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciDHAF272564:GCV8-2546-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction."
    Kim S.H., Harzman C., Davis J.K., Hutcheson R., Broderick J.B., Marsh T.L., Tiedje J.M.
    BMC Microbiol. 12:21-21(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DCB-2 / DSM 10664.

Entry informationi

Entry nameiTHIM_DESHD
AccessioniPrimary (citable) accession number: B8FUD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: July 22, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.