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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfitobacterium hafniense (strain DCB-2 / DSM 10664)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1
Metal bindingi892ZincUniRule annotation1
Metal bindingi895ZincUniRule annotation1
Metal bindingi912ZincUniRule annotation1
Metal bindingi915ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDHAF272564:G1GUH-4150-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Dhaf_4041
OrganismiDesulfitobacterium hafniense (strain DCB-2 / DSM 10664)
Taxonomic identifieri272564 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfitobacterium
Proteomesi
  • UP000007726 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891511 – 922Isoleucine--tRNA ligaseAdd BLAST922

Proteomic databases

PRIDEiB8FT35

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB8FT35
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B8FT35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYRNTLNLP ETDFPMRGNL PQREPEILQK WEEEDIYATV QKARAGRPKF
60 70 80 90 100
VLHDGPPYAN GDIHLGHALN KVIKDIIVKY KTMAGFDAPY VPGWDTHGLP
110 120 130 140 150
IEQQVIKKLG VNRHAVSVVE FRRMCKEYAK KYISIQKEQF KRLGVRGDWQ
160 170 180 190 200
NPYLTLEKEY EAAQIGVFGK MARKKYIYKG LKPVYWCPSC ETALAEAEIE
210 220 230 240 250
YAEKTSHAIY VKFPVKEGKG VLTDENTFVI IWTTTPWTLP ANLAITLHEE
260 270 280 290 300
FSYVQVQVEK EHWLVAEGML ESLRSLWNLE LPVEKRFVGK ELEGVICKHP
310 320 330 340 350
FIERDSVLIL GEHVTLEAGT GCVHTAPGHG EEDFNVGKKY GLPVLCPVDH
360 370 380 390 400
QGKFTAEGGA YAGMKVDKAN PVIIEDLKNL HALVHEDKIK HSYAHCWRCN
410 420 430 440 450
NPIIYRATEQ WFASIDGFRK AALEEIDKVQ WIPSWGKDRI YNMIADRGDW
460 470 480 490 500
CISRQRTWGV PIPIFYCEDC GKEIISDETI AKVQEIFREE GSDAWFLRPA
510 520 530 540 550
AELLPEGFTC ACGGKSFRKE TDIMDVWFDS GTSHTSVLME RKELAWPADL
560 570 580 590 600
YMEGSDQHRG WFNSSLSTSV AAYGKAPYKA VLTHGFLVDE KGRKMSKSLG
610 620 630 640 650
NGVDPLQVTK EMGADILRLW VCAADYKNDV AVSPRIMKQM SEAYRKIRNT
660 670 680 690 700
LRFLLSNLND FDPAKDRVAY KDLPEIDRWA LLQLGKVTQR VLQGYEKYEF
710 720 730 740 750
HWVYHSVHNF CAVELSAIYL DIVKDRLYVE GKNSTLRRAS QTVLYDVLNA
760 770 780 790 800
LVRLMAPVLT YTADEIWPYV PGVPAGSHVQ TEEMPEALPQ WLDEALEKKW
810 820 830 840 850
DTLLAVRSEV TKALEKARQD KLINHPLTAQ VDLYPNAELE GFLRGIPNLS
860 870 880 890 900
EIFIVSAVQL HSAGEEKPEG LSMAEDLAGF GIAVNSAAGE KCERCWIYDT
910 920
GVGENQEHPT LCPRCASVVS HL
Length:922
Mass (Da):104,696
Last modified:March 3, 2009 - v1
Checksum:iB643CE2C0E98E9F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001336 Genomic DNA Translation: ACL22051.1
RefSeqiWP_015944977.1, NC_011830.1

Genome annotation databases

EnsemblBacteriaiACL22051; ACL22051; Dhaf_4041
KEGGidhd:Dhaf_4041

Similar proteinsi

Entry informationi

Entry nameiSYI_DESHD
AccessioniPrimary (citable) accession number: B8FT35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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