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B8FT35

- SYI_DESHD

UniProt

B8FT35 - SYI_DESHD

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Dhaf_4041
Organism
Desulfitobacterium hafniense (strain DCB-2 / DSM 10664)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylate By similarity
Binding sitei597 – 5971ATP By similarity
Metal bindingi892 – 8921Zinc By similarity
Metal bindingi895 – 8951Zinc By similarity
Metal bindingi912 – 9121Zinc By similarity
Metal bindingi915 – 9151Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDHAF272564:GCV8-4117-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Dhaf_4041
OrganismiDesulfitobacterium hafniense (strain DCB-2 / DSM 10664)
Taxonomic identifieri272564 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfitobacterium
ProteomesiUP000007726: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 922922Isoleucine--tRNA ligaseUniRule annotationPRO_1000189151Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272564.Dhaf_4041.

Structurei

3D structure databases

ProteinModelPortaliB8FT35.
SMRiB8FT35. Positions 1-922.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8FT35-1 [UniParc]FASTAAdd to Basket

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MDYRNTLNLP ETDFPMRGNL PQREPEILQK WEEEDIYATV QKARAGRPKF    50
VLHDGPPYAN GDIHLGHALN KVIKDIIVKY KTMAGFDAPY VPGWDTHGLP 100
IEQQVIKKLG VNRHAVSVVE FRRMCKEYAK KYISIQKEQF KRLGVRGDWQ 150
NPYLTLEKEY EAAQIGVFGK MARKKYIYKG LKPVYWCPSC ETALAEAEIE 200
YAEKTSHAIY VKFPVKEGKG VLTDENTFVI IWTTTPWTLP ANLAITLHEE 250
FSYVQVQVEK EHWLVAEGML ESLRSLWNLE LPVEKRFVGK ELEGVICKHP 300
FIERDSVLIL GEHVTLEAGT GCVHTAPGHG EEDFNVGKKY GLPVLCPVDH 350
QGKFTAEGGA YAGMKVDKAN PVIIEDLKNL HALVHEDKIK HSYAHCWRCN 400
NPIIYRATEQ WFASIDGFRK AALEEIDKVQ WIPSWGKDRI YNMIADRGDW 450
CISRQRTWGV PIPIFYCEDC GKEIISDETI AKVQEIFREE GSDAWFLRPA 500
AELLPEGFTC ACGGKSFRKE TDIMDVWFDS GTSHTSVLME RKELAWPADL 550
YMEGSDQHRG WFNSSLSTSV AAYGKAPYKA VLTHGFLVDE KGRKMSKSLG 600
NGVDPLQVTK EMGADILRLW VCAADYKNDV AVSPRIMKQM SEAYRKIRNT 650
LRFLLSNLND FDPAKDRVAY KDLPEIDRWA LLQLGKVTQR VLQGYEKYEF 700
HWVYHSVHNF CAVELSAIYL DIVKDRLYVE GKNSTLRRAS QTVLYDVLNA 750
LVRLMAPVLT YTADEIWPYV PGVPAGSHVQ TEEMPEALPQ WLDEALEKKW 800
DTLLAVRSEV TKALEKARQD KLINHPLTAQ VDLYPNAELE GFLRGIPNLS 850
EIFIVSAVQL HSAGEEKPEG LSMAEDLAGF GIAVNSAAGE KCERCWIYDT 900
GVGENQEHPT LCPRCASVVS HL 922
Length:922
Mass (Da):104,696
Last modified:March 3, 2009 - v1
Checksum:iB643CE2C0E98E9F2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001336 Genomic DNA. Translation: ACL22051.1.
RefSeqiWP_015944977.1. NC_011830.1.
YP_002460487.1. NC_011830.1.

Genome annotation databases

EnsemblBacteriaiACL22051; ACL22051; Dhaf_4041.
GeneIDi7261061.
KEGGidhd:Dhaf_4041.
PATRICi21666091. VBIDesHaf15223_4202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001336 Genomic DNA. Translation: ACL22051.1 .
RefSeqi WP_015944977.1. NC_011830.1.
YP_002460487.1. NC_011830.1.

3D structure databases

ProteinModelPortali B8FT35.
SMRi B8FT35. Positions 1-922.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272564.Dhaf_4041.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL22051 ; ACL22051 ; Dhaf_4041 .
GeneIDi 7261061.
KEGGi dhd:Dhaf_4041.
PATRICi 21666091. VBIDesHaf15223_4202.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DHAF272564:GCV8-4117-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction."
    Kim S.H., Harzman C., Davis J.K., Hutcheson R., Broderick J.B., Marsh T.L., Tiedje J.M.
    BMC Microbiol. 12:21-21(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DCB-2 / DSM 10664.

Entry informationi

Entry nameiSYI_DESHD
AccessioniPrimary (citable) accession number: B8FT35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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