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B8FR00

- PNP_DESHD

UniProt

B8FR00 - PNP_DESHD

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, Dhaf_3670
Organism
Desulfitobacterium hafniense (strain DCB-2 / DSM 10664)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi491 – 4911Magnesium By similarity
Metal bindingi497 – 4971Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciDHAF272564:GCV8-3747-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Dhaf_3670
OrganismiDesulfitobacterium hafniense (strain DCB-2 / DSM 10664)
Taxonomic identifieri272564 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfitobacterium
ProteomesiUP000007726: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 723723Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_1000185733Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272564.Dhaf_3670.

Structurei

3D structure databases

ProteinModelPortaliB8FR00.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini558 – 61760KHAdd
BLAST
Domaini627 – 70175S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8FR00-1 [UniParc]FASTAAdd to Basket

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MTQEVLERSI QVGGRTMTFQ TGKIGKQAGG AIFCRYGDTV VSAFATGSAQ    50
PREGIDFFPL TVEFEERLYA AGKIPGGFIK REGRPSEKAI LSARLIDRPI 100
RPLFPEGYRN DVQVVAQVMS VDQDCASDIT GINAASAALT ISNVPFEGPV 150
AAVTVGLIGD EFIINPTVEQ SEKSVMHLTV AGTKDAVMMV EAGAQEVPEA 200
QMLEAIMFGH REIQRIAEFI ENYRLEALER NLAKPKQEVV MKQMPEEIVQ 250
AVKAFAYDKM DQAVRTEEKK AREEAIRQVK EEALAHFAEQ YPEDLKTIDK 300
ILEDFVHKIV RRLITVEHIR PDGRALDEIR PISVEVGILP RTHGTGLFTR 350
GQTQVLTVAT LGAVGDEQIL DGLGLEESKR YMHHYNFPPY SVGETRPMRG 400
PGRREIGHGA LAERALLPVI PNENDFPYTI RLVSEVLESN GSSSMASVCG 450
STLSLMDAGV PVKSPVAGIA MGLISEENHI AILSDIQGME DHDGDMDFKV 500
AGTSQGVTAL QMDIKIKGVS REILERALTQ AKEGRLFILD KMLSVIEKPR 550
PELSPFAPRI ITASIHPDKI REVIGPGGKT IKKIIDETGV KIDIEDDGRV 600
FISAVDGEAG ENALKIIQAL TQEVEVGRIY NGRVTRIMDF GAFVEVIPGV 650
LGLSGKEGLV HISQLAHGRV EKVEDVVKLG DEILVKATGI DKQGRLNLSR 700
KEALPNPNPN PNGSTANKNP RNS 723
Length:723
Mass (Da):78,989
Last modified:March 3, 2009 - v1
Checksum:i375248A85BDFE56A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001336 Genomic DNA. Translation: ACL21687.1.
RefSeqiYP_002460123.1. NC_011830.1.

Genome annotation databases

EnsemblBacteriaiACL21687; ACL21687; Dhaf_3670.
GeneIDi7260691.
KEGGidhd:Dhaf_3670.
PATRICi21665325. VBIDesHaf15223_3818.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001336 Genomic DNA. Translation: ACL21687.1 .
RefSeqi YP_002460123.1. NC_011830.1.

3D structure databases

ProteinModelPortali B8FR00.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272564.Dhaf_3670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL21687 ; ACL21687 ; Dhaf_3670 .
GeneIDi 7260691.
KEGGi dhd:Dhaf_3670.
PATRICi 21665325. VBIDesHaf15223_3818.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci DHAF272564:GCV8-3747-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction."
    Kim S.H., Harzman C., Davis J.K., Hutcheson R., Broderick J.B., Marsh T.L., Tiedje J.M.
    BMC Microbiol. 12:21-21(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DCB-2 / DSM 10664.

Entry informationi

Entry nameiPNP_DESHD
AccessioniPrimary (citable) accession number: B8FR00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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