B8FQZ6 (DUT_DESHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	Dhaf_3666

Organism

Desulfitobacterium hafniense (strain DCB-2 / DSM 10664) [Complete proteome] [HAMAP]

Taxonomic identifier

272564 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfitobacterium

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_1000119231
Regions
	Region	70 – 72	3	Substrate binding By similarity
	Region	87 – 89	3	Substrate binding By similarity
Sites
	Binding site	83	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8FQZ6 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: F6CC04F2930F12E1	FASTA	151	15,812
	10 20 30 40 50 60 MNSIKVKIAY VRKDKAPKLP QYATPGAAGV DLQASLDQEL TIEPGQIVKI PTGLAIELPH 70 80 90 100 110 120 AGVGAFVFAR SGLASKYGLA LANGVGVIDS DYRGEILVAV INQGSEPFVV KDGDRIAQMV 130 140 150 FLPVFIGEFY LADQLDETGR GCGGFGSTGV S

References

[1]

"Complete sequence of Desulfitobacterium hafniense DCB-2." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Madsen T., Christiansen N., Ahring B., Marsh T., Harzman C., Davis J.K., Kim S.-H., Tiedje J.M. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DCB-2 / DSM 10664.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001336 Genomic DNA. Translation: ACL21683.1.
RefSeq	YP_002460119.1. NC_011830.1.
3D structure databases
ProteinModelPortal	B8FQZ6.
ModBase	Search...
Protein-protein interaction databases
STRING	B8FQZ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7260687.
GenomeReviews	Gene locus Dhaf_3666 in contig CP001336_GR.
KEGG	dhd:Dhaf_3666.
PATRIC	21665317. VBIDesHaf15223_3814.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG436079.
OMA	LDLRACI.
ProtClustDB	CLSK2466295.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_DESHD

Accession

Primary (citable) accession number: B8FQZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program