B8FIX5 (LEXA_DESAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LexA repressor
EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	Dalk_2674

Organism

Desulfatibacillum alkenivorans (strain AK-01) [Complete proteome] [HAMAP]

Taxonomic identifier

439235 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfobacterales › Desulfobacteraceae › Desulfatibacillum › Desulfatibacillum alkenivorans

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP-Rule MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP-Rule MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	LexA repressor HAMAP-Rule MF_00015		PRO_1000201818
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	122	1	For autocatalytic cleavage activity By similarity
	Active site	159	1	For autocatalytic cleavage activity By similarity
	Site	87 – 88	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8FIX5 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: B2487F121E4AFA89	FASTA	203	22,404
	10 20 30 40 50 60 MEDLTEKQSL VFEFIMEYTA DHGYPPTVRE LCDELGFKSP NTAHFHLKGL KDKGYIQSAK 70 80 90 100 110 120 GKNRGITVLK TPPGAGGKIP LVGRIAAGAP ILAVENVMDT LDVDRAFFGS SDAFSVRVEG 130 140 150 160 170 180 DSMIEAHIED GDYVVIKPTA TPRNGDIVAA LVNDEVTLKY FHRDGSRIEL RPANVRYKPF 190 200 CYTEEDFIDV RVLGVMAGLI RKV

References

[1]

"Complete sequence of Desulfatibacillum alkenivorans AK-01." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Wawrik B., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AK-01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001322 Genomic DNA. Translation: ACL04366.1.
RefSeq	YP_002431834.1. NC_011768.1.
3D structure databases
ProteinModelPortal	B8FIX5.
ModBase	Search...
Protein-protein interaction databases
STRING	439235.Dalk_2674.
Protein family/group databases
MEROPS	S24.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL04366; ACL04366; Dalk_2674.
GeneID	7166609.
KEGG	dal:Dalk_2674.
PATRIC	21652028. VBIDesAlk8144_2866.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HOG000232167.
KO	K01356.
OMA	RGDSMKN.
ProtClustDB	CLSK2805429.
Enzyme and pathway databases
BioCyc	DALK439235:GHP2-2702-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 2.10.109.10. 1 hit.
HAMAP	MF_00015. LexA.
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_DESAA

Accession

Primary (citable) accession number: B8FIX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program