B8FIN2 (ACSA_DESAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	Dalk_2329

Organism

Desulfatibacillum alkenivorans (strain AK-01) [Complete proteome] [HAMAP]

Taxonomic identifier

439235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfobacterales › Desulfobacteraceae › Desulfatibacillum

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000137263
Sites
	Active site	513	1	By similarity
Amino acid modifications
	Modified residue	605	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8FIN2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: D6591BCE2A709DD2	FASTA	646	72,114
	10 20 30 40 50 60 MSNNLFTVPE DWQKRAWCDD ARYQKMYDES VNDPDAFWSR EAQRIDWFEP FSKVKNSSFN 70 80 90 100 110 120 GDVDIKWFLD GKLNVAYNCL DRHLEKRGDQ TAIIFEGNEP GVEEKITYRQ LYERVCRFSN 130 140 150 160 170 180 VLKSCGVKKG DRVSIYLPMI PQLAVAMLAC ARIGAIHSIV FAGFSPDALA NRITDAECTI 190 200 210 220 230 240 LITADEGLRG PKAIGLKEAA DEAMDKAGMV KKCIVVKHTG ADVPMKAGRD LWWHELTAKE 250 260 270 280 290 300 STECPPESMD SEDPLFILYT SGSTGTPKGV LHTTGGYIVY TSLTHQYVFD YHDGDIYWCT 310 320 330 340 350 360 ADIGWVTGHS YIIYGPLANG AVTIMFEGIP NYPDFSRFWQ ICDKHQVNIF YTAPTVVRAL 370 380 390 400 410 420 MQQGDEPVKA TSRTSVRLLG TVGEPINPEA WLWYHRVVGE ERCPIVDTWW QTETGGIMIT 430 440 450 460 470 480 PLPGATALKP GSATRPFFGI QPALIDKEGN QLEGPGEGYL VMLDSWPALA RTVYGDHRRF 490 500 510 520 530 540 KSTYFIQCPG TYFSGDGARR DEDGYYWITG RIDDVINVSG HRLGTAEIES SLVAHPAVSE 550 560 570 580 590 600 AAVVGFPHDI KGQAIYAYVT LSSGYEPSEE LRKELSLFVR KDIGPIATPE VLQFTDSLPK 610 620 630 640 TRSGKIMRRI LRKVACNELD NLGDTSTLAD PAVVEALIKN RIDACR

References

[1]

"Complete sequence of Desulfatibacillum alkenivorans AK-01." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Wawrik B., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AK-01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001322 Genomic DNA. Translation: ACL04022.1.
RefSeq	YP_002431490.1. NC_011768.1.
3D structure databases
ProteinModelPortal	B8FIN2.
SMR	B8FIN2. Positions 8-641.
ModBase	Search...
Protein-protein interaction databases
STRING	B8FIN2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7166261.
GenomeReviews	Gene locus Dalk_2329 in contig CP001322_GR.
KEGG	dal:Dalk_2329.
PATRIC	21651286. VBIDesAlk8144_2498.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	TRGTEES.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_DESAA

Accession

Primary (citable) accession number: B8FIN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program