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B8FHQ1

- BIOB_DESAA

UniProt

B8FHQ1 - BIOB_DESAA

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Protein
Biotin synthase
Gene
bioB, Dalk_0763
Organism
Desulfatibacillum alkenivorans (strain AK-01)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi111 – 1111Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi202 – 2021Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciDALK439235:GHP2-769-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Dalk_0763
OrganismiDesulfatibacillum alkenivorans (strain AK-01)
Taxonomic identifieri439235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfatibacillum
ProteomesiUP000000739: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Biotin synthaseUniRule annotation
PRO_0000381344Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi439235.Dalk_0763.

Structurei

3D structure databases

ProteinModelPortaliB8FHQ1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8FHQ1-1 [UniParc]FASTAAdd to Basket

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MDLKKCLDMA EAAKAGQLPE ISDLEELACL PEQHVLDLLP AANAVRKHFF    50
GDQVHLCCIV NGKSGKCRED CAFCAQSAYA KTSAPVYPLI DSEKMFQGAS 100
YARENGVHRY SLVASGGRLP KAEVRMVAEA FSRMGGDGVG YCASLGILDA 150
QDFALLKEAG VDRYHHNLET AESHFKEICT THAYGDRVKT IREAKKAGMS 200
VCAGGLFGIG ESDLQVLELG LALRDLEVDS VPVNFLIPIK GTRLEKSSDL 250
SPLRCLKIIS LLRFALGGKE IVICGGRESN LEELHPLVFY AGASGIMTGD 300
YLTAPGRNPQ KDHAMIRRLG LTALREGE 328
Length:328
Mass (Da):35,528
Last modified:March 3, 2009 - v1
Checksum:iE108F9413520C678
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001322 Genomic DNA. Translation: ACL02468.1.
RefSeqiWP_012609907.1. NC_011768.1.
YP_002429936.1. NC_011768.1.

Genome annotation databases

EnsemblBacteriaiACL02468; ACL02468; Dalk_0763.
GeneIDi7164676.
KEGGidal:Dalk_0763.
PATRICi21647880. VBIDesAlk8144_0814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001322 Genomic DNA. Translation: ACL02468.1 .
RefSeqi WP_012609907.1. NC_011768.1.
YP_002429936.1. NC_011768.1.

3D structure databases

ProteinModelPortali B8FHQ1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 439235.Dalk_0763.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL02468 ; ACL02468 ; Dalk_0763 .
GeneIDi 7164676.
KEGGi dal:Dalk_0763.
PATRICi 21647880. VBIDesAlk8144_0814.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci DALK439235:GHP2-769-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AK-01.

Entry informationi

Entry nameiBIOB_DESAA
AccessioniPrimary (citable) accession number: B8FHQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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