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B8FHB6 (B8FHB6_DESAA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Valine--tRNA ligase HAMAP-Rule MF_02004
EC=6.1.1.9 HAMAP-Rule MF_02004
Alternative name(s):
Valyl-tRNA synthetase HAMAP-Rule MF_02004
Gene names
Name:valS HAMAP-Rule MF_02004
Ordered Locus Names:Dalk_0497
OrganismDesulfatibacillum alkenivorans (strain AK-01) [Complete proteome] [HAMAP] EMBL ACL02204.1
Taxonomic identifier439235 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Desulfobacterales › Desulfobacteraceae › Desulfatibacillum ›

Protein attributes

Sequence length888 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004 SAAS SAAS019499

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02004 SAAS SAAS019499.

Domain

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. HAMAP-Rule MF_02004

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Coiled coil810 – 88576 By similarity HAMAP-Rule MF_02004
Motif46 – 5611"HIGH" region By similarity HAMAP-Rule MF_02004
Motif524 – 5285"KMSKS" region By similarity HAMAP-Rule MF_02004

Sites

Binding site5271ATP By similarity HAMAP-Rule MF_02004

Sequences

Sequence LengthMass (Da)Tools
B8FHB6 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: E49142980C2DAAB9

FASTA888100,749
        10         20         30         40         50         60 
MDSKLDKGYE PHDVEAHWYA FWESEGLFAA KEEGGDNTYS IVIPPPNVTG VLHMGHALNV 

        70         80         90        100        110        120 
TLQDILCRYR RSKGDNVLWM PGTDHAGIAT QNVVERQLAS EGTDRHQVGR EAFIERVWEW 

       130        140        150        160        170        180 
RKQYGGAIIN QLKRLGASCD WDRERFTMDE GLSRAVRKVF VDLYDQGLIY RGDYIINWCP 

       190        200        210        220        230        240 
RCHTALADLE VEHEQTDGKL YQILYPFVDG EGGLIVATTR PETMLGDTAV AINPEDERYA 

       250        260        270        280        290        300 
DLTAEKVMLP IMNREIPIVK DAYVDLKFGT GALKVTPAHD VNDFEIGLRH DLSRVKVIGE 

       310        320        330        340        350        360 
DGCMTAEAGE FEGMERFECR KAIVAKLDEM GLLKKVESHD HGVGQCYRCK TTVEPNLSKQ 

       370        380        390        400        410        420 
WFVKAGPLAE KAIKAVETKK TRIVPEVWEK TYFEWLYNIR DWCISRQIWW GHRIPAWTCQ 

       430        440        450        460        470        480 
DCGEIMVRME DPDKCTCGST NLEQESDVLD TWFSSALWPF STMGWPDQTQ LLKAYYPTSV 

       490        500        510        520        530        540 
LITGFDILFF WVARMMMMGL QFMDDVPFKD VYIHALVRDE EGKKMSKSKG NVIDPLEVIE 

       550        560        570        580        590        600 
SQGTDAFRFT LASFAAMGRD IKLSVKRIEG YRHFINKLWN AARFTLTNVE NNPDAAAKAS 

       610        620        630        640        650        660 
KSRAIPDRWI LSRLNQVILQ VEESLEAYQF NEAANILYKF VWHEFCDWYL EAIKPILYRE 

       670        680        690        700        710        720 
GAQEERNATV ATLQRVLKDA IVLLHPFIPF VTEEIWHHMP GAEGSIMKAP YPAAVDEAVG 

       730        740        750        760        770        780 
LGPDKEAERE MELVMAVVTA VRNIRGEMNI PPNQALAAII EPTDDFAKEA LARESGLVSN 

       790        800        810        820        830        840 
LARLSSLEVG GKRPKGAATA VVDGASVYVL LEGVIDLDKE EARLEKEIAK LTKELVKLDK 

       850        860        870        880 
KLNNEDFLAK APDKVVEKVR GQQAEMTEKR EHLESNLEKV REIHGDQG

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References

[1]"Complete sequence of Desulfatibacillum alkenivorans AK-01."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Wawrik B., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AK-01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001322 Genomic DNA. Translation: ACL02204.1.
RefSeqYP_002429672.1. NC_011768.1.

3D structure databases

ProteinModelPortalB8FHB6.
ModBaseSearch...

Protein-protein interaction databases

STRING439235.Dalk_0497.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL02204; ACL02204; Dalk_0497.
GeneID7164409.
KEGGdal:Dalk_0497.
PATRIC21647312. VBIDesAlk8144_0531.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0525.
HOGENOMHOG000020094.
KOK01873.
OMAIMDALIR.
ProtClustDBPRK05729.

Enzyme and pathway databases

BioCycDALK439235:GHP2-502-MONOMER.

Family and domain databases

Gene3D1.10.287.380. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8FHB6_DESAA
AccessionPrimary (citable) accession number: B8FHB6
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info