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B8FGM5 (B8FGM5_DESAA) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Dalk_3244 EMBL ACL04934.1
OrganismDesulfatibacillum alkenivorans (strain AK-01) [Complete proteome] [HAMAP] EMBL ACL04934.1
Taxonomic identifier439235 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfatibacillum

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Potential EMBL ACL04934.1
Chain16 – 320305 Potential EMBL ACL04934.1
PRO_5000735621

Regions

Domain103 – 305203ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding131 – 18656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2591Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2721Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2721Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2741Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
B8FGM5 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 4DBEF56669C1284D

FASTA32034,246
        10         20         30         40         50         60 
MKRLNLALLF GGASAEREVS ILSGTQVLNA LDKDRFIVRC YDPKIDIPRL VHDAPNIDVA 

        70         80         90        100        110        120 
VVMLHGEYGE DGRIQGLLDM LDIPYQGSGV LGSAVSMSKL ASKQLYEQAG IKTPPYRVVS 

       130        140        150        160        170        180 
PDNRHEAANL ADQVGYPLFV KPCMGGSSIG MSLVKGPADL DKALDAGFAE DPVLLLEACV 

       190        200        210        220        230        240 
TGVEITGAVL GNDELEALPL IEIVPGEEFA FFEYGAKYEA GKSEEICPAR ISEEGAQKAM 

       250        260        270        280        290        300 
DLSKQAHKAL FCKGYSRTDM ILDKDGALHV LETNTIPGMT ANSLLPKAAK VAGMDFTALM 

       310        320 
SRLVDLALEA KGRPGHIETA 

« Hide

References

[1]"Complete sequence of Desulfatibacillum alkenivorans AK-01."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Wawrik B., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AK-01 EMBL ACL04934.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001322 Genomic DNA. Translation: ACL04934.1.
RefSeqYP_002432402.1. NC_011768.1.

3D structure databases

ProteinModelPortalB8FGM5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING439235.Dalk_3244.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL04934; ACL04934; Dalk_3244.
GeneID7167186.
KEGGdal:Dalk_3244.
PATRIC21653238. VBIDesAlk8144_3465.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAKYTEGFA.
OrthoDBEOG6ND0KB.
ProtClustDBCLSK928465.

Enzyme and pathway databases

BioCycDALK439235:GHP2-3279-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8FGM5_DESAA
AccessionPrimary (citable) accession number: B8FGM5
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)