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B8FCY5 (IF2_DESAA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:Dalk_4738
OrganismDesulfatibacillum alkenivorans (strain AK-01) [Complete proteome] [HAMAP]
Taxonomic identifier439235 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfatibacillum

Protein attributes

Sequence length1040 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processsmall GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10401040Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000190631

Regions

Nucleotide binding548 – 5558GTP By similarity
Nucleotide binding594 – 5985GTP By similarity
Nucleotide binding648 – 6514GTP By similarity
Region542 – 690149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
B8FCY5 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: B612112D07102DB3

FASTA1,040111,922
        10         20         30         40         50         60 
MAKLRVHELA RELNMTNKVL LDKLQAMDLP TPVRSHMSSL DDDVVALVKA SLVRPKAKSV 

        70         80         90        100        110        120 
VEKRIGTTVI RRRKKVVKPA EGGPEETAKP EEGAEEGVQE MDAAQESVSA PEAEKAPEPV 

       130        140        150        160        170        180 
AEKAPEPQKA APAAEEPKAE EGAPAPEKAA PVQAKEEPEE APQAEAKPAP PKKVDVTPPG 

       190        200        210        220        230        240 
DKAKIIAKPE PPQPAEPEPP KEEPAPVELP EEKQAVSAKA ADTPAEPQEE PEAKPEIKAE 

       250        260        270        280        290        300 
AKAEEGAPEK PAEEPKAKEE QKAAPEDSKE EPKAEEPAQP AEDEKAEEKA KAPEEKEPAK 

       310        320        330        340        350        360 
SQEPQAAKEP SKEAPKPKGK KKKKKETAAK IISLPTRPVE PVVPPASRQK PAPQSPAGPG 

       370        380        390        400        410        420 
GPGGPGVPPQ PRAPQDLGGD PAAKKEKRKK KGRRDGGTTE ETDSKFFKKK ISFRKKAVVE 

       430        440        450        460        470        480 
GADLYDKTPG KLRKGKKGGK AQPVRQQKTQ TTTPKAIKRR IKIDEAIAVA DLAKRLGIKA 

       490        500        510        520        530        540 
AELIKNLMGM GIMATVNQML DYDTAVLLAA EFGYEVEKAN FEEETVLKAE EDAPETLSNR 

       550        560        570        580        590        600 
PPVVTIMGHV DHGKTSLLDA IRESNVTGGE AGGITQHIGA YLVDSPRGRI AFLDTPGHEA 

       610        620        630        640        650        660 
FTAMRARGAQ VTDIVILVVA ADDGVMPQTV EAVNHSKAAG VPIIVAVNKM DKEGADPDRV 

       670        680        690        700        710        720 
MRELSDHGLV PEDWGGDTIF VQVSAKQRQG LDDLLEMVLL QAEVLELQAN PDKLARGHVV 

       730        740        750        760        770        780 
EAKVDPGRGP VATVLIQEGT LHAGDVVVCG IHYGKVRAMY NEKRQPLDAA GPACPAEILG 

       790        800        810        820        830        840 
LSGVPMAGDE IIAVDDEKVA KQVSSHRAQK QRATELAKSS KLSLDNLFDQ MQAGNVKDLN 

       850        860        870        880        890        900 
LIIKADVQGS IEALRESLEK LSGDEVKIHV VHAATGTVLE SDVALAAVSN AIIVAFNVRP 

       910        920        930        940        950        960 
SPKVSDMAAE EHVDIRFYDI IYNAINEIKD AIVGLMESTY KEHVSGRAEV RDTFTIPKVG 

       970        980        990       1000       1010       1020 
TIAGCYVTDG KVERHNQVRL LRDGVVIFDG KLSSLRRFKD DVKEVATGYE CGMGIENFND 

      1030       1040 
IKIGDVLELY YLEEIKPVLK 

« Hide

References

[1]"Complete sequence of Desulfatibacillum alkenivorans AK-01."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Wawrik B., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AK-01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001322 Genomic DNA. Translation: ACL06416.1.
RefSeqYP_002433884.1. NC_011768.1.

3D structure databases

ProteinModelPortalB8FCY5.
SMRB8FCY5. Positions 940-1035.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING439235.Dalk_4738.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL06416; ACL06416; Dalk_4738.
GeneID7168708.
KEGGdal:Dalk_4738.
PATRIC21656382. VBIDesAlk8144_5012.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076906.
KOK02519.
OMAFECFEME.
OrthoDBEOG67HJSV.

Enzyme and pathway databases

BioCycDALK439235:GHP2-4801-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SUPFAMSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_DESAA
AccessionPrimary (citable) accession number: B8FCY5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries