Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B8F9F0 (SYL_DESAA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Dalk_1193
OrganismDesulfatibacillum alkenivorans (strain AK-01) [Complete proteome] [HAMAP]
Taxonomic identifier439235 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfatibacillum

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199195

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B8F9F0 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: CEDE2E1693FC4A01

FASTA86398,398
        10         20         30         40         50         60 
MDERYDPKKI EGKWQAHWDA AGLFKVKEDP SKEKYFLMEM FPYPSGKIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVARFKRMQG FNVLHPMGWD AFGMPAENAA IKNNTHPAKW TYENIAAMGA QLKAMGFSYD 

       130        140        150        160        170        180 
WDREIATCKP DYYRWEQWLF VQMFKKGMVY RKEASVNWCH TCQTVLANEQ VEQNMCWRCG 

       190        200        210        220        230        240 
DEVEQKKLKQ WFFRITDYAD DLLEHCDKLP GWPEKVVTMQ KNWIGKSHGA EILFSVKDSD 

       250        260        270        280        290        300 
VKIKVFTTRP DTLCGATFMC LAPEHPLVED LSKGTAQEAV VQEFVARMAK QDKAKRTADD 

       310        320        330        340        350        360 
KEKEGVPIGA QCINPLTGKT MEIYTANFAL MEYGTGAVMS VPTHDQRDFE FATKYGLEKI 

       370        380        390        400        410        420 
VVIQPEGEAL SPETMTEAYT DPGVLVNSGQ FDGMKNTDAM EAIAQYLDEN DMGKKTVQYR 

       430        440        450        460        470        480 
IRDWGISRQR YWGAPIPMIH CPDCGITPVP EDQLPVILPE DANLLEGGKS PLPELDSFVK 

       490        500        510        520        530        540 
TTCPQCGNEN ARRETDTMDT FVESSWYPER YCSPKCDDGM FDVDAVKYWM PVDQYIGGVE 

       550        560        570        580        590        600 
HAVMHLLYSR YFTRVLNDFG LVDFKEPFTR LLTQGMVCKE TLKCPEHGWL FPHEVEGSGD 

       610        620        630        640        650        660 
ERTCTKCGKI VEAGRVEKMS KSKLNVVDPE ELLAKYGADT IRLFCLFAAP PERDLDWSEE 

       670        680        690        700        710        720 
GVEGSYRFLQ RVWRMFAAHM DEVKDAQPFE GSPDDLDGYL KDLYKKTHQT IKKVTEDIDG 

       730        740        750        760        770        780 
RFHFNTAISA VMELVNMVYG LDDNLQGEKR AGVLRAAVEA VILLISPMTP HFTEELWSLF 

       790        800        810        820        830        840 
GKTQCVLETP WPAWREDALT ADEVVVVLQV NGKLRSKLNV PLDTDDEKIK EMALADEKVQ 

       850        860 
KFMGGKPIRK VIVVKNKLVN VVV 

« Hide

References

[1]"Complete sequence of Desulfatibacillum alkenivorans AK-01."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Wawrik B., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AK-01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001322 Genomic DNA. Translation: ACL02896.1.
RefSeqYP_002430364.1. NC_011768.1.

3D structure databases

ProteinModelPortalB8F9F0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING439235.Dalk_1193.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL02896; ACL02896; Dalk_1193.
GeneID7165107.
KEGGdal:Dalk_1193.
PATRIC21648786. VBIDesAlk8144_1266.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycDALK439235:GHP2-1200-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_DESAA
AccessionPrimary (citable) accession number: B8F9F0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries