B8F7U6 (FMT_HAEPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	HAPS_1910

Organism

Haemophilus parasuis serovar 5 (strain SH0165) [Complete proteome] [HAMAP]

Taxonomic identifier

557723 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus parasuis

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000190027
Regions
	Region	112 – 115	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8F7U6 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: EC8605A30B29109D	FASTA	316	34,676
	10 20 30 40 50 60 MKKLNIIFAG TPDFAATHLQ ALLNSEHNVI AVYTQPDKPA GRGKKLQASP VKQLAEVHHI 70 80 90 100 110 120 PVYQPKSLRK EEAQAELQAL NADVMVVVAY GLILPEAVLK APKYGCLNVH GSLLPRWRGA 130 140 150 160 170 180 APIQRSIWAG DTETGVTIMQ MDIGLDTGDM LHKVTTPILA TETSASLYAK LAELAPPALL 190 200 210 220 230 240 EVLNGLTSGQ FKPEKQQDEQ ANYAEKLTKE EAKLDWNMTA CQLERNIRAF NPAPMAYLTL 250 260 270 280 290 300 MVNEVEERIK VYQAEVLPHQ EKTVGTVLAV DKNGIQIATQ QGVLNITQLQ PAGKKPMSVQ 310 DFLNGRGDWF KVGSVL

References

[1]	"Complete genome sequence of Haemophilus parasuis SH0165." Yue M., Yang F., Yang J., Bei W., Cai X., Chen L., Dong J., Zhou R., Jin M., Jin Q., Chen H. J. Bacteriol. 191:1359-1360(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SH0165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001321 Genomic DNA. Translation: ACL33398.1.
RefSeq	YP_002476346.1. NC_011852.1.
3D structure databases
ProteinModelPortal	B8F7U6.
ModBase	Search...
Protein-protein interaction databases
STRING	557723.HAPS_1910.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACL33398; ACL33398; HAPS_1910.
GeneID	7278756.
KEGG	hap:HAPS_1910.
PATRIC	20196162. VBIHaePar127548_1895.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	HPAR557723:GH24-1910-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_HAEPS

Accession

Primary (citable) accession number: B8F7U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program