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B8F7K2

- NAPA_HAEPS

UniProt

B8F7K2 - NAPA_HAEPS

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Haemophilus parasuis serovar 5 (strain SH0165)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciHPAR557723:GH24-1794-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:HAPS_1794
OrganismiHaemophilus parasuis serovar 5 (strain SH0165)
Taxonomic identifieri557723 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000006743: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 827795Periplasmic nitrate reductasePRO_1000186365Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi557723.HAPS_1794.

Structurei

3D structure databases

ProteinModelPortaliB8F7K2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiAGKMHDS.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8F7K2-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MELNRRDFMK ANAAAAAALA AGITLPVKNV YANDNSIKWD KAPCRFCGTG
60 70 80 90 100
CSVLVGTQNG RMVASQGDPD AEVNRGLNCI KGYFLPKIIY GKDRLTSPML
110 120 130 140 150
RMKDGKYDKN GEFTPVSWDQ AFTIMAEKFK KAIKEQGPNG VGMFTSGQST
160 170 180 190 200
IFEGVAKSKL FKAGLRSNNI DPNARHCMAS AAVAFMRTFG MDEPMGCYDD
210 220 230 240 250
IEKADAFVLW GSNMAEMHPI LWSRISDRRL SKQDAKVAVL STFEHRSFEL
260 270 280 290 300
ADLPIVFKPQ GDLAIMNYIA NYLIQNNAID QDFIQKHTKF KRGETDIGYG
310 320 330 340 350
LRDNHPLEQA AKNAKTAGKM HDSSFEEFKQ LVSEYTLDKA HELSGVPKDQ
360 370 380 390 400
LESLAKMYAD PNLNIVSFWT MGFNQHVRGV WANHLIYNIH LLTGKISKPG
410 420 430 440 450
CGPFSLTGQP SACGTAREVG TFIHRLPADL VVTNPEHVKT AEKLWKLPNG
460 470 480 490 500
VIQTKVGLHA VAQDRALKDG KLNAYWVMCN NNMQCGPNIN QERMPGWRDE
510 520 530 540 550
RNFIVVSDPY PTVSALSADL ILPTAMWVEK EGAYGNAERR TQFWFQQVKA
560 570 580 590 600
PGEAKSDLWQ LVEFSKYFTT DEMWPAEVLA ANPEYKGKTL FDVLYRNGQV
610 620 630 640 650
DKFQVPADKA GYINDEADHF GFYLQKGLFE EYAEFGRGHG HDLASFETYH
660 670 680 690 700
KARGLRWPVV DGKETLWRYR EGYDPYVQAG EGVSFYGNKD KRAVILAVPY
710 720 730 740 750
EPPAEAPDAE YDLWLSTGRV LEHWHSGSMT RRVPELHRSF PNNLVWMHPT
760 770 780 790 800
DAQKRGLRHG DKVKVISRRG EMISHLDTRG RNKVPEGLIY TTFFDAGQLA
810 820
NKLTLDATDP ISKETDFKKC AVKVVKA
Length:827
Mass (Da):92,969
Last modified:March 3, 2009 - v1
Checksum:i6722FA2A30F7A16A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001321 Genomic DNA. Translation: ACL33304.1.
RefSeqiYP_002476252.1. NC_011852.1.

Genome annotation databases

EnsemblBacteriaiACL33304; ACL33304; HAPS_1794.
GeneIDi7277707.
KEGGihap:HAPS_1794.
PATRICi20195911. VBIHaePar127548_1775.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001321 Genomic DNA. Translation: ACL33304.1 .
RefSeqi YP_002476252.1. NC_011852.1.

3D structure databases

ProteinModelPortali B8F7K2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 557723.HAPS_1794.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACL33304 ; ACL33304 ; HAPS_1794 .
GeneIDi 7277707.
KEGGi hap:HAPS_1794.
PATRICi 20195911. VBIHaePar127548_1775.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi AGKMHDS.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci HPAR557723:GH24-1794-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Haemophilus parasuis SH0165."
    Yue M., Yang F., Yang J., Bei W., Cai X., Chen L., Dong J., Zhou R., Jin M., Jin Q., Chen H.
    J. Bacteriol. 191:1359-1360(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SH0165.

Entry informationi

Entry nameiNAPA_HAEPS
AccessioniPrimary (citable) accession number: B8F7K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3