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B8F7K2

- NAPA_HAEPS

UniProt

B8F7K2 - NAPA_HAEPS

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Haemophilus parasuis serovar 5 (strain SH0165)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciHPAR557723:GH24-1794-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:HAPS_1794
    OrganismiHaemophilus parasuis serovar 5 (strain SH0165)
    Taxonomic identifieri557723 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000006743: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
    BLAST
    Chaini33 – 827795Periplasmic nitrate reductasePRO_1000186365Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi557723.HAPS_1794.

    Structurei

    3D structure databases

    ProteinModelPortaliB8F7K2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiAGKMHDS.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B8F7K2-1 [UniParc]FASTAAdd to Basket

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    MELNRRDFMK ANAAAAAALA AGITLPVKNV YANDNSIKWD KAPCRFCGTG    50
    CSVLVGTQNG RMVASQGDPD AEVNRGLNCI KGYFLPKIIY GKDRLTSPML 100
    RMKDGKYDKN GEFTPVSWDQ AFTIMAEKFK KAIKEQGPNG VGMFTSGQST 150
    IFEGVAKSKL FKAGLRSNNI DPNARHCMAS AAVAFMRTFG MDEPMGCYDD 200
    IEKADAFVLW GSNMAEMHPI LWSRISDRRL SKQDAKVAVL STFEHRSFEL 250
    ADLPIVFKPQ GDLAIMNYIA NYLIQNNAID QDFIQKHTKF KRGETDIGYG 300
    LRDNHPLEQA AKNAKTAGKM HDSSFEEFKQ LVSEYTLDKA HELSGVPKDQ 350
    LESLAKMYAD PNLNIVSFWT MGFNQHVRGV WANHLIYNIH LLTGKISKPG 400
    CGPFSLTGQP SACGTAREVG TFIHRLPADL VVTNPEHVKT AEKLWKLPNG 450
    VIQTKVGLHA VAQDRALKDG KLNAYWVMCN NNMQCGPNIN QERMPGWRDE 500
    RNFIVVSDPY PTVSALSADL ILPTAMWVEK EGAYGNAERR TQFWFQQVKA 550
    PGEAKSDLWQ LVEFSKYFTT DEMWPAEVLA ANPEYKGKTL FDVLYRNGQV 600
    DKFQVPADKA GYINDEADHF GFYLQKGLFE EYAEFGRGHG HDLASFETYH 650
    KARGLRWPVV DGKETLWRYR EGYDPYVQAG EGVSFYGNKD KRAVILAVPY 700
    EPPAEAPDAE YDLWLSTGRV LEHWHSGSMT RRVPELHRSF PNNLVWMHPT 750
    DAQKRGLRHG DKVKVISRRG EMISHLDTRG RNKVPEGLIY TTFFDAGQLA 800
    NKLTLDATDP ISKETDFKKC AVKVVKA 827
    Length:827
    Mass (Da):92,969
    Last modified:March 3, 2009 - v1
    Checksum:i6722FA2A30F7A16A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001321 Genomic DNA. Translation: ACL33304.1.
    RefSeqiWP_010786603.1. NC_011852.1.
    YP_002476252.1. NC_011852.1.

    Genome annotation databases

    EnsemblBacteriaiACL33304; ACL33304; HAPS_1794.
    GeneIDi7277707.
    KEGGihap:HAPS_1794.
    PATRICi20195911. VBIHaePar127548_1775.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001321 Genomic DNA. Translation: ACL33304.1 .
    RefSeqi WP_010786603.1. NC_011852.1.
    YP_002476252.1. NC_011852.1.

    3D structure databases

    ProteinModelPortali B8F7K2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 557723.HAPS_1794.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL33304 ; ACL33304 ; HAPS_1794 .
    GeneIDi 7277707.
    KEGGi hap:HAPS_1794.
    PATRICi 20195911. VBIHaePar127548_1775.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi AGKMHDS.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci HPAR557723:GH24-1794-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Haemophilus parasuis SH0165."
      Yue M., Yang F., Yang J., Bei W., Cai X., Chen L., Dong J., Zhou R., Jin M., Jin Q., Chen H.
      J. Bacteriol. 191:1359-1360(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SH0165.

    Entry informationi

    Entry nameiNAPA_HAEPS
    AccessioniPrimary (citable) accession number: B8F7K2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3