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B8F5N0 (NAGZ_HAEPS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:HAPS_1013
OrganismHaemophilus parasuis serovar 5 (strain SH0165) [Complete proteome] [HAMAP]
Taxonomic identifier557723 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_1000133665

Regions

Region157 – 1582Substrate binding By similarity

Sites

Active site1701Proton donor/acceptor By similarity
Active site2421Nucleophile By similarity
Binding site601Substrate By similarity
Binding site681Substrate By similarity
Binding site1271Substrate By similarity
Site1681Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
B8F5N0 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: BC39F92576C5391B

FASTA34038,363
        10         20         30         40         50         60 
MLLIDLAGYE LTQEEQELLE HPLVSGLILF TRNFHDKAQL QALIKSVRQR VKKPLLITVD 

        70         80         90        100        110        120 
QEGGRVQRFR EGFTKLPAMQ SFLQLDRLQL AQEAGWLMSA EMFALDIDLS FAPVLDLGHC 

       130        140        150        160        170        180 
SKAIGDRSFG EDVATMLPVA EAFIDGMREI GMAATGKHFP GHGHVIADSH LETPFDDRPK 

       190        200        210        220        230        240 
ETIFNHDIQP FKQLIAKGKL SAIMPAHVIY TQCDSQPASG SSYWLKEILR KQLQFNGVIF 

       250        260        270        280        290        300 
SDDLGMKGAG FMGNYVERSE KALQAGCDLL LLCNEPDGVV QVLDNLKYQP TPTQKERYLS 

       310        320        330        340 
LMKRKQISWS ELTASPRWQQ AHQQLATLQD QWLEWKAQNA 

« Hide

References

[1]"Complete genome sequence of Haemophilus parasuis SH0165."
Yue M., Yang F., Yang J., Bei W., Cai X., Chen L., Dong J., Zhou R., Jin M., Jin Q., Chen H.
J. Bacteriol. 191:1359-1360(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SH0165.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001321 Genomic DNA. Translation: ACL32632.1.
RefSeqYP_002475580.1. NC_011852.1.

3D structure databases

ProteinModelPortalB8F5N0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557723.HAPS_1013.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL32632; ACL32632; HAPS_1013.
GeneID7277081.
KEGGhap:HAPS_1013.
PATRIC20194309. VBIHaePar127548_1011.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAAGFSSYW.
OrthoDBEOG6BCT06.
ProtClustDBPRK05337.

Enzyme and pathway databases

BioCycHPAR557723:GH24-1013-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_HAEPS
AccessionPrimary (citable) accession number: B8F5N0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries