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B8F5G6 (RUVC_HAEPS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:HAPS_0941
OrganismHaemophilus parasuis serovar 5 (strain SH0165) [Complete proteome] [HAMAP]
Taxonomic identifier557723 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length192 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 192192Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000195260

Sites

Metal binding81Magnesium By similarity
Metal binding671Magnesium By similarity
Metal binding1391Magnesium By similarity
Metal binding1421Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B8F5G6 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 8FE8BB49C7FFF5BD

FASTA19220,918
        10         20         30         40         50         60 
MAIILGIDPG SRVTGYGVIR QTGRHLEYLG SGAIRTSVED LPTRLKRIYA GVTEIITQFQ 

        70         80         90        100        110        120 
PDMFAIEQVF MAKNADSALK LGQARGTAIV AAVNHDLPVF EYAARLVKQT VVGIGSADKV 

       130        140        150        160        170        180 
QVQDMVTRIL QLSAKPQADA ADALAIAITH AHSIQHSLTV AKQSSQAVSS EKEQILALMK 

       190 
TRYSRGRFRF RN 

« Hide

References

[1]"Complete genome sequence of Haemophilus parasuis SH0165."
Yue M., Yang F., Yang J., Bei W., Cai X., Chen L., Dong J., Zhou R., Jin M., Jin Q., Chen H.
J. Bacteriol. 191:1359-1360(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SH0165.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001321 Genomic DNA. Translation: ACL32568.1.
RefSeqYP_002475516.1. NC_011852.1.

3D structure databases

ProteinModelPortalB8F5G6.
SMRB8F5G6. Positions 2-158.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557723.HAPS_0941.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACL32568; ACL32568; HAPS_0941.
GeneID7277814.
KEGGhap:HAPS_0941.
PATRIC20194155. VBIHaePar127548_0938.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAILHMIKL.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycHPAR557723:GH24-941-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_HAEPS
AccessionPrimary (citable) accession number: B8F5G6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families