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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Haemophilus parasuis serovar 5 (strain SH0165) (Glaesserella parasuis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei562Aminoacyl-adenylateUniRule annotation1
Binding sitei606ATPUniRule annotation1
Metal bindingi901ZincUniRule annotation1
Metal bindingi904ZincUniRule annotation1
Metal bindingi921ZincUniRule annotation1
Metal bindingi924ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPAR557723:G1GUF-909-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HAPS_0916
OrganismiHaemophilus parasuis serovar 5 (strain SH0165) (Glaesserella parasuis)
Taxonomic identifieri557723 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeGlaesserella
Proteomesi
  • UP000006743 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891681 – 938Isoleucine--tRNA ligaseAdd BLAST938

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi557723.HAPS_0916

Structurei

3D structure databases

ProteinModelPortaliB8F5E2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi603 – 607"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiPSWYIRT

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B8F5E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKNTLNL PETGFPMRGD LAKREPAMLQ NWHDKKLYQK IREASKGKRS
60 70 80 90 100
FILHDGPPYA NGNIHLGHAV NHILKDIINK SKTAMGFDTP YVPGWDCHGL
110 120 130 140 150
PIELKVEGLV GKPNENISAS EFRQKCREYA KEQVDGQKAD FMRLGILGDW
160 170 180 190 200
DNPYLTMNFD TEAHIIRTLG KVIANGHLYK GSKPVHWCLD CGSSLAEAEV
210 220 230 240 250
EYEDKVSPSI YVRFSAVDPV AVEAKFNAQG KGSGQISAVI WTTTPWTLPS
260 270 280 290 300
NRAIALNSEL EYQLVQFGDE RVILATELVE AVQKVTGVEQ VEVLGSAKGS
310 320 330 340 350
DLELMRFNHP FYDFSVPFIL GDHVTTDGGT GLVHTAPDHG LDDYIVGQKY
360 370 380 390 400
KLEMAGLVAN DGKFISTTPF FAGKGVFETN DLVLEKLKET GALLKLERIK
410 420 430 440 450
HSYPHCWRHK TPIIFRATPQ WFIGMETQGL RKQALGEIKR VRWIPSWGEA
460 470 480 490 500
RIDTMVANRP DWCISRQRTW GVPMTMFVHN ETEQLHPRTL EILEEVAKRV
510 520 530 540 550
EQAGIQAWWD LDPKEVLGEE DAKIYRKVPD TLDVWFDSGS TYASVVQQRP
560 570 580 590 600
EFNGNSADMY LEGSDQHRGW FMSSLMLSTA TDNKAPYNQV LTHGFTVDEK
610 620 630 640 650
GRKMSKSLGN VIVPSEVWNK NGADILRLWV ASTDYTGEIA VSHNILNSAG
660 670 680 690 700
DTYRRIRNTA RFLLANLNGF DPKRDLVKPE EMIALDRWAV SCALDAQNDI
710 720 730 740 750
KEAYDNYQFH TVVQRLMRFC SIEMGSFYLD IIKDRQYTTK ADSLARRSCQ
760 770 780 790 800
TALWHISEAL VRWIAPILSF TADEIWGYLP QVEGRSEFVF TEEFYTDLFG
810 820 830 840 850
LSESDKLDDN YWQKLLKVRA EVNRVLEQAR NDKLIGAALE AKVTVYANDD
860 870 880 890 900
IRPLLEQLGN ELGFVLITSQ AIVKPLAEAD VAEGELAGLA VKVERADGEK
910 920 930
CPRCWHYATD IGANAEHAEI CGRCVENVVG EGETRNFA
Length:938
Mass (Da):105,815
Last modified:March 3, 2009 - v1
Checksum:iCAF030ADACA7BC38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001321 Genomic DNA Translation: ACL32544.1
RefSeqiWP_012622009.1, NC_011852.1

Genome annotation databases

EnsemblBacteriaiACL32544; ACL32544; HAPS_0916
GeneIDi7277067
KEGGihap:HAPS_0916
PATRICifig|557723.8.peg.914

Similar proteinsi

Entry informationi

Entry nameiSYI_HAEPS
AccessioniPrimary (citable) accession number: B8F5E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 25, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health