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Protein

DNA topoisomerase 3

Gene

topB

Organism
Haemophilus parasuis serovar 5 (strain SH0165) (Glaesserella parasuis)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Magnesium 1; catalyticUniRule annotation1
Metal bindingi103Magnesium 1; catalyticUniRule annotation1
Metal bindingi103Magnesium 2UniRule annotation1
Metal bindingi105Magnesium 2UniRule annotation1
Active sitei329O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciHPAR557723:G1GUF-429-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotationImported
Ordered Locus Names:HAPS_0428Imported
OrganismiHaemophilus parasuis serovar 5 (strain SH0165) (Glaesserella parasuis)Imported
Taxonomic identifieri557723 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeGlaesserella
Proteomesi
  • UP000006743 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Interaction with DNAUniRule annotation1
Sitei170Interaction with DNAUniRule annotation1
Sitei178Interaction with DNAUniRule annotation1
Sitei185Interaction with DNAUniRule annotation1
Sitei331Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi557723.HAPS_0428

Structurei

3D structure databases

ProteinModelPortaliB8F447
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 134ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 199Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IYH Bacteria
COG0550 LUCA
HOGENOMiHOG000086848
KOiK03169
OMAiTYPRVDT

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00953 Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005738 TopoIII
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01056 topB, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

B8F447-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFIAEKPS LGRAIADVLP KPHTRGDGFI QCGEDNVVTW CIGHLLEQAE
60 70 80 90 100
PDAYDERFKM WRMEHLPIIP EQWKLIPKKE TLKQFKVVER LIKQADILVN
110 120 130 140 150
AGDPDREGQL LVDEVFGYLN LSPERKSQIQ RCLISDLNPN AVKKAIEKLQ
160 170 180 190 200
FNRDFVPLAT SALARARADW LYGINMTRAY TLQGRWAGYK GVLSVGRVQT
210 220 230 240 250
PVLGLIVRRD LEIENFIPKD YFEVLANVVV PETQERFQAQ WQPSKACEDY
260 270 280 290 300
QDEDGRVLSR PLAENVVKRI TDQAACVTEY QDKMESEAAP LPYSLSALQI
310 320 330 340 350
DAARRFGLSA QDVLDVCQRL YETHKLITYP RSDNRYLPNE HFADRLKVMA
360 370 380 390 400
AIAHHLPEYA QKPDAVDPNK KNRCWNDSKV EAHHAIIPTA RQGRQVHLTQ
410 420 430 440 450
NEQWIYQLIA RQYLMQFCPD AEYRKGKIVL NIAGGTFIAQ ARNLVVAGWK
460 470 480 490 500
SLLGKEDNDE IVEPLLPVVK KDQQLWCESG EIISKKTQPP RHFTDATLLS
510 520 530 540 550
AMTGIARFVQ DKQLKKVLRE TDGLGTEATR AGIIELLFKR GFLIKKGRNI
560 570 580 590 600
HSTEAGRTLI SALPDVATQP DMTAHWEMQL TDISKKQASY QQFMADLNQR
610 620 630 640
LPSLLSSPNK QILQSLAKIT PLTTSSKSRY TRKSTQNVEK
Length:640
Mass (Da):72,886
Last modified:March 3, 2009 - v1
Checksum:i9DE8AF6F5F744D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001321 Genomic DNA Translation: ACL32099.1
RefSeqiWP_012621725.1, NC_011852.1

Genome annotation databases

EnsemblBacteriaiACL32099; ACL32099; HAPS_0428
GeneIDi7276987
KEGGihap:HAPS_0428
PATRICifig|557723.8.peg.432

Similar proteinsi

Entry informationi

Entry nameiB8F447_HAEPS
AccessioniPrimary (citable) accession number: B8F447
Entry historyiIntegrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: April 25, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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