B8F3Q0 (MUKB_HAEPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosome partition protein MukB

Alternative name(s):
Structural maintenance of chromosome-related protein

Gene names

Name:	mukB
Ordered Locus Names:	HAPS_0263

Organism

Haemophilus parasuis serovar 5 (strain SH0165) [Complete proteome] [HAMAP]

Taxonomic identifier

557723 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	1496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a central role in chromosome condensation, segregation and cell cycle progression. Functions as a homodimer, which is essential for chromosome partition. Involved in negative DNA supercoiling in vivo, and by this means organize and compact chromosomes. May achieve or facilitate chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division By similarity. HAMAP MF_01800
Subunit structure	Homodimerization via its hinge domain. Binds to DNA via its C-terminal region. Interacts, and probably forms a ternary complex, with MukE and MukF via its C-terminal region. The complex formation is stimulated by calcium or magnesium. Interacts with tubulin-related protein FtsZ By similarity.
Subcellular location	Cytoplasm › nucleoid By similarity. Note: Restricted to the nucleoid region By similarity. HAMAP MF_01800
Domain	The hinge domain, which separates the large intramolecular coiled coil regions, allows the homodimerization, forming a V-shaped homodimer By similarity. HAMAP MF_01800
Sequence similarities	Belongs to the SMC family. MukB subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition DNA condensation
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome condensation Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nucleoid Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1496	1496	Chromosome partition protein MukB HAMAP MF_01800		PRO_1000187479
Regions
	Nucleotide binding	62 – 69	8	ATP Potential
	Region	694 – 811	118	Flexible hinge By similarity
	Coiled coil	326 – 492	167	Potential
	Coiled coil	534 – 632	99	Potential
	Coiled coil	863 – 942	80	Potential
	Coiled coil	1005 – 1171	167	Potential
	Coiled coil	1235 – 1291	57	Potential

Sequences

	Sequence		Length	Mass (Da)
	B8F3Q0 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 8FA763645FD2EBCD	FASTA	1,496	171,744
	10 20 30 40 50 60 MTEKNDLFEQ NVTSQNIMVA THVQSSIPSV SRSKFRSLTL INWNGFFART FDLDELVTTL 70 80 90 100 110 120 SGGNGAGKST TMAGFVTALI PDLTLLNFRN TTEAGSTSSS RDKGLYGKLK AGVCYAVLES 130 140 150 160 170 180 ENSRGQRVIS GVRLQQIAGR DKKVDIRSFS LQNINENQSV ISILTEQVGE KNARVLALND 190 200 210 220 230 240 LKEKFDGSEV QFKQYHSITD YHSFLFDLGV IPKRLRTSGD RSKFYKLIEA SLYGGISSVI 250 260 270 280 290 300 TKSLRDYLLP ENTGVRQAFQ DMESALRENR MTLEAIKVTQ SDRDMFKKLI TESTNYVSAD 310 320 330 340 350 360 YMRHANERRG NVQQAIEKRQ EWYQSKSKLT LEQQRLVEFS REMQDLEQGE SSLEAEYNSA 370 380 390 400 410 420 SDHLNLVMNA VRHQEKVERY QDDVAELNER LEEQQMALEE VAERQEMAQA RATEAEDQVE 430 440 450 460 470 480 ELRSQMADYQ QALDAQQTRA LQYQQAVNAL EKAKQISGLA NLDLHNVEDY HAEFVAQADE 490 500 510 520 530 540 ITDKVFELEQ RLSVSDMAKS QFDKAYELVC KIAGETDRLQ ADSVARELLS AYPSQKAHAQ 550 560 570 580 590 600 QAVALRQKLT ELEQRLHQQQ NAERLLAEFN QKAQAELENA EDLESYYEEQ QARLEDLEAE 610 620 630 640 650 660 LAEFVEVRST QRQQREQLNQ QYQQLAQTAP AWHTAQSALA RLQEQCGETF DSSQAVMQFM 670 680 690 700 710 720 QNTLSREREA TLERDELARR EQLLDEQISR LSQPDGAEDI RLNQLAEKFG GVLISELYED 730 740 750 760 770 780 VSIEDAPYFS ALYGDARHAI VVRNLEAVKA QLQQLDDCPE NLYLIEGDPN AFDDNVFKSE 790 800 810 820 830 840 ELGDGVVVQL SDRQWRYSKF SEFAVFGRAS REKQLEKVKA ERDETTEKHA ERAFDVQKCQ 850 860 870 880 890 900 RLHQHLSQFV GTHLALAFQP DPEVAMQEIA QKRAEIEREL NQASGTEQQL RHQLENSKAQ 910 920 930 940 950 960 LQLLNKVLPQ LNILADETLQ DRVEECREQL LEAQEDEQFI RQFGNALAQL EPIAVALKSD 970 980 990 1000 1010 1020 PTQFEQLQAD YKRSVEQQKL QQQKVFALAD VMNRRLHFSY QETVGAEGSA LNEQLRQRLD 1030 1040 1050 1060 1070 1080 NAQREREQAR EQLRQATAQF SEYNQVLTSL RSAFDAKNQM LQELLQEMDE YGIRNDDGAE 1090 1100 1110 1120 1130 1140 ERARIRRDEL QQRLSQHRMR KSYIDKQVAV IQAEMDNLNK AVRKAERDYH TQREIVVQAK 1150 1160 1170 1180 1190 1200 VSWCLVLKLS RNSDVEKRLN RRELAYQSAE ELRSISDKAL GALRTAVADN EYLRDSLRAS 1210 1220 1230 1240 1250 1260 EDSRKPENKV VFFILVYQHL RERIRQDIIK TDDPIDAIEQ MEIELSRLTN ELTSREKKLA 1270 1280 1290 1300 1310 1320 ISSESVANIL RKTIQREQNR ILQLNQGLQN IAFGQVKGVR LVVNIRDTHA ILLNALSNDR 1330 1340 1350 1360 1370 1380 EQHSDLFENQ KLSFSEALAM LYKRVNPHIE LGQRTPQTIG EELLDYRNYL DLEVETFRGA 1390 1400 1410 1420 1430 1440 DGWMRAESSA LSTGEAIGTG MSILLMVVQS WEEESRRMRA KDILPARLLF LDEAARLDAT 1450 1460 1470 1480 1490 SINTLFELCE RLDMQLLIAA PENISPERGT TYKLVRKITN NQEYVHVVGL KGFGRV

References

[1]	"Complete genome sequence of Haemophilus parasuis SH0165." Yue M., Yang F., Yang J., Bei W., Cai X., Chen L., Dong J., Zhou R., Jin M., Jin Q., Chen H. J. Bacteriol. 191:1359-1360(2009) [PubMed: 19074396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SH0165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001321 Genomic DNA. Translation: ACL31952.1.
RefSeq	YP_002474900.1. NC_011852.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8F3Q0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7277450.
GenomeReviews	Gene locus HAPS_0263 in contig CP001321_GR.
KEGG	hap:HAPS_0263.
PATRIC	20192801. VBIHaePar127548_0270.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296838.
OMA	RGFANEI.
ProtClustDB	PRK04863.
Family and domain databases
HAMAP	MF_01800. MukB. [Tree]
InterPro	IPR012090. MukB. IPR007406. Scp_MukB_N. [Graphical view]
Gene3D	G3DSA:3.40.1140.10. Scp_MukB_N. 1 hit.
KO	K03632.
PANTHER	PTHR18937:SF146. PTHR18937:SF146. 1 hit.
Pfam	PF04310. MukB. 1 hit. [Graphical view]
PIRSF	PIRSF005246. MukB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MUKB_HAEPS

Accession

Primary (citable) accession number: B8F3Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program