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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Haemophilus parasuis serovar 5 (strain SH0165) (Glaesserella parasuis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi254Magnesium or manganese 1UniRule annotation1
Metal bindingi267Magnesium or manganese 1UniRule annotation1
Metal bindingi267Magnesium or manganese 2UniRule annotation1
Metal bindingi269Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi132 – 187ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:HAPS_0121
OrganismiHaemophilus parasuis serovar 5 (strain SH0165) (Glaesserella parasuis)
Taxonomic identifieri557723 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeGlaesserella
Proteomesi
  • UP000006743 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001897371 – 303D-alanine--D-alanine ligaseAdd BLAST303

Interactioni

Protein-protein interaction databases

STRINGi557723.HAPS_0121

Structurei

3D structure databases

ProteinModelPortaliB8F3B8
SMRiB8F3B8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 300ATP-graspUniRule annotationAdd BLAST197

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011592
KOiK01921
OMAiMQGLLEC

Family and domain databases

Gene3Di3.30.1490.201 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

B8F3B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLKNEKIAV LFGGVSAERE ISLNSGKSVF EALQSLGYNV EAIDTKEFPI
60 70 80 90 100
EKLKEKGIQR VFNILHGGIG ENGVLQGALE QLGIPYTGCG VMASAITLDK
110 120 130 140 150
FRTKLLWNAV GLPTAAMEVV RRGQAVNSDE IIAKLGLPLF VKPASEGSSV
160 170 180 190 200
GVSKVKTAEQ LLPAIEEALK YDSIVLVEEN LAGAEYSVPV LDGEVLPAVQ
210 220 230 240 250
IIPDGEFYDY HAKYISDNTQ YVVPALNADR QAEVAKLVKQ AYDVVGCRGW
260 270 280 290 300
SRIDVMEDGE GNFRLVEVNT CPGMTSHSIF PKSAATVGYS FERLVERVLE

LSA
Length:303
Mass (Da):32,782
Last modified:March 3, 2009 - v1
Checksum:i50C500BEB2C5F362
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001321 Genomic DNA Translation: ACL31820.1
RefSeqiWP_005711197.1, NC_011852.1

Genome annotation databases

EnsemblBacteriaiACL31820; ACL31820; HAPS_0121
GeneIDi7277963
KEGGihap:HAPS_0121

Similar proteinsi

Entry informationi

Entry nameiDDL_HAEPS
AccessioniPrimary (citable) accession number: B8F3B8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 25, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome