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Protein

Argininosuccinate synthase

Gene

argG

Organism
Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei94CitrullineUniRule annotation1
Binding sitei99CitrullineUniRule annotation1
Binding sitei124ATP; via amide nitrogenUniRule annotation1
Binding sitei126AspartateUniRule annotation1
Binding sitei130AspartateUniRule annotation1
Binding sitei130CitrullineUniRule annotation1
Binding sitei131AspartateUniRule annotation1
Binding sitei134CitrullineUniRule annotation1
Binding sitei185CitrullineUniRule annotation1
Binding sitei194CitrullineUniRule annotation1
Binding sitei270CitrullineUniRule annotation1
Binding sitei282CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 22ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSIL395965:G1GUA-1418-MONOMER
UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Msil_1382
OrganismiMethylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002016861 – 416Argininosuccinate synthaseAdd BLAST416

Proteomic databases

PRIDEiB8ESL2

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi395965.Msil_1382

Structurei

3D structure databases

ProteinModelPortaliB8ESL2
SMRiB8ESL2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OMAiGVGRIDM
OrthoDBiPOG091H01U0

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.90.1260.10, 1 hit
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

B8ESL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSKQSDIKK VVLAYSGGLD TSIILKWLET TYGCEVVTFT ADLGQGEEIE
60 70 80 90 100
PARQKAILLG VKPENIFIED LREEFVRDYV FPMFRANAQY EGLYLLGTSI
110 120 130 140 150
ARPLIAKKQI EIAKKTGADA VAHGATGKGN DQVRFELSYY ALDPDIKVIA
160 170 180 190 200
PWREWDLTSR TALIAFAEQH QIPIAKDKRG DAPFSVDANL LHASSEGKVL
210 220 230 240 250
EDPAEEVPDY VYSRTVNPED APDKPTYIEI DFERGDPVAI DGEALSPANL
260 270 280 290 300
LTRLNELARV NGVGRLDLVE NRFVGMKSRG MYETPGGTIL YVAHRGIESI
310 320 330 340 350
TLDRGAAHLK DELAPKYSEL IYNGFWFSPE REMLQALIDK SQEFVAGRVR
360 370 380 390 400
VKLYKGSAVV VGRSSPYSLY DQDLVTFEEG AVAYDHRDAA GFIKLNALRL
410
RTLGKRARKI AAAQKA
Length:416
Mass (Da):46,217
Last modified:March 3, 2009 - v1
Checksum:i79ADAB8398051ECA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA Translation: ACK50347.1
RefSeqiWP_012590417.1, NC_011666.1

Genome annotation databases

EnsemblBacteriaiACK50347; ACK50347; Msil_1382
KEGGimsl:Msil_1382

Entry informationi

Entry nameiASSY_METSB
AccessioniPrimary (citable) accession number: B8ESL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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