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B8ESL2 (ASSY_METSB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:Msil_1382
OrganismMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP]
Taxonomic identifier395965 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000201686

Regions

Nucleotide binding14 – 229ATP By similarity

Sites

Binding site411ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site941Citrulline By similarity
Binding site991Citrulline By similarity
Binding site1241ATP; via amide nitrogen By similarity
Binding site1261Aspartate By similarity
Binding site1301Aspartate By similarity
Binding site1301Citrulline By similarity
Binding site1311Aspartate By similarity
Binding site1341Citrulline By similarity
Binding site1851Citrulline By similarity
Binding site1941Citrulline By similarity
Binding site2701Citrulline By similarity
Binding site2821Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
B8ESL2 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 79ADAB8398051ECA

FASTA41646,217
        10         20         30         40         50         60 
MPSKQSDIKK VVLAYSGGLD TSIILKWLET TYGCEVVTFT ADLGQGEEIE PARQKAILLG 

        70         80         90        100        110        120 
VKPENIFIED LREEFVRDYV FPMFRANAQY EGLYLLGTSI ARPLIAKKQI EIAKKTGADA 

       130        140        150        160        170        180 
VAHGATGKGN DQVRFELSYY ALDPDIKVIA PWREWDLTSR TALIAFAEQH QIPIAKDKRG 

       190        200        210        220        230        240 
DAPFSVDANL LHASSEGKVL EDPAEEVPDY VYSRTVNPED APDKPTYIEI DFERGDPVAI 

       250        260        270        280        290        300 
DGEALSPANL LTRLNELARV NGVGRLDLVE NRFVGMKSRG MYETPGGTIL YVAHRGIESI 

       310        320        330        340        350        360 
TLDRGAAHLK DELAPKYSEL IYNGFWFSPE REMLQALIDK SQEFVAGRVR VKLYKGSAVV 

       370        380        390        400        410 
VGRSSPYSLY DQDLVTFEEG AVAYDHRDAA GFIKLNALRL RTLGKRARKI AAAQKA 

« Hide

References

[1]"Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001280 Genomic DNA. Translation: ACK50347.1.
RefSeqYP_002361709.1. NC_011666.1.

3D structure databases

ProteinModelPortalB8ESL2.
SMRB8ESL2. Positions 10-401.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395965.Msil_1382.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK50347; ACK50347; Msil_1382.
GeneID7091720.
KEGGmsl:Msil_1382.
PATRIC22598267. VBIMetSil55537_1511.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAAPPEEAY.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycMSIL395965:GCND-1407-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_METSB
AccessionPrimary (citable) accession number: B8ESL2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways