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B8EQP4

- SYL_METSB

UniProt

B8EQP4 - SYL_METSB

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Protein

Leucine--tRNA ligase

Gene
leuS, Msil_0336
Organism
Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei633 – 6331ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSIL395965:GCND-342-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:Msil_0336
OrganismiMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
ProteomesiUP000002257: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Leucine--tRNA ligaseUniRule annotationPRO_1000199214Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi395965.Msil_0336.

Structurei

3D structure databases

ProteinModelPortaliB8EQP4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionUniRule annotationAdd
BLAST
Motifi630 – 6345"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHISHELW.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8EQP4-1 [UniParc]FASTAAdd to Basket

« Hide

MDFERYNARE TEPKWRDKWE RSATFKTDND DPRPKYYVLE MFPYPSGRIH    50
MGHVRNYTMG DVIARYKRAR GFSVLHPMGW DAFGMPAENA AMQNKSHPAA 100
WTYANIEAMR AQLKSMGLSL DWSREIATCD PAYYRHQQKM FLDFLAAGLV 150
DRKKSKVNWD PVDHTVLANE QVIDGRGWRS GALVEQRELT QWFFKITDYA 200
EDLLRSLDGL TRWPEKVRLM QANWIGRSEG LLVRFALEPT LSAPATEIEV 250
YTTRPDTLFG AKFLAIAADH PIAADCAKAD PALAAFIAEC RLRGTSVAAI 300
ETAEKKGVDT GLRVRHPFDE NWLLPVYVAN FVLMDYGTGA IFGCPAHDQR 350
DLDFANAYGL GATPVILPPD ADPKTFVIAD KAYDGDGLLF HSRFLDGMTV 400
EAAQEEVARR LEEHPLGNRP QAKRQVNFRL RDWGISRQRY WGCPIPIIHC 450
PEHGAVPVPA KDLPVELPPD VSFDEPGNPL DRHPTWKHVN CPICAAPSLR 500
ETDTMDTFVD SSWYFARFTQ PWMEDAPTDP SAIAKWLPVD QYIGGVEHAI 550
LHLLYARFFT RAMQATGHAG EIREPFAGLF TQGMVVHETY RSEAGDWMFP 600
ADVRIEIGPA GRRAFDLASG AEVSIGGIEK MSKSKRNTVD PDEIIGTFGA 650
DTARWFVLSD SPPERDVIWT EEGVQGAAKF VQRLWRLIGE LIFLSGADGA 700
PKPQSISPQA AAIRRAAHQS LIRLEEDLDR LRFNRAVAQA HDLANKLGAA 750
VGDIDSVEIA PDLRFAFREA AEILTLMIAP MMPHLAEECW ARLGHKGLAA 800
EAPWPQADHS LVVEETIDLP VQVNGKKRGD LIIDRAAGRE AIEAAALALE 850
PVKRALEGRP VKKIIIVPQR IVNVVA 876
Length:876
Mass (Da):97,986
Last modified:March 3, 2009 - v1
Checksum:iD68C834EEF4747A5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001280 Genomic DNA. Translation: ACK49315.1.
RefSeqiYP_002360677.1. NC_011666.1.

Genome annotation databases

EnsemblBacteriaiACK49315; ACK49315; Msil_0336.
GeneIDi7091555.
KEGGimsl:Msil_0336.
PATRICi22595947. VBIMetSil55537_0369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001280 Genomic DNA. Translation: ACK49315.1 .
RefSeqi YP_002360677.1. NC_011666.1.

3D structure databases

ProteinModelPortali B8EQP4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395965.Msil_0336.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK49315 ; ACK49315 ; Msil_0336 .
GeneIDi 7091555.
KEGGi msl:Msil_0336.
PATRICi 22595947. VBIMetSil55537_0369.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200747.
KOi K01869.
OMAi HISHELW.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci MSIL395965:GCND-342-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
    Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
    J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BL2 / DSM 15510 / NCIMB 13906.

Entry informationi

Entry nameiSYL_METSB
AccessioniPrimary (citable) accession number: B8EQP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi