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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei633ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSIL395965:G1GUA-344-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Msil_0336
OrganismiMethylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
Proteomesi
  • UP000002257 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001992141 – 876Leucine--tRNA ligaseAdd BLAST876

Proteomic databases

PRIDEiB8EQP4

Interactioni

Protein-protein interaction databases

STRINGi395965.Msil_0336

Structurei

3D structure databases

ProteinModelPortaliB8EQP4
SMRiB8EQP4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 53"HIGH" regionAdd BLAST11
Motifi630 – 634"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T Bacteria
COG0495 LUCA
HOGENOMiHOG000200747
KOiK01869
OMAiTFMVLAP
OrthoDBiPOG091H01JZ

Family and domain databases

Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_00049_B Leu_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR002302 Leu-tRNA-ligase
IPR025709 Leu_tRNA-synth_edit
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR43740 PTHR43740, 1 hit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 3 hits
PF13603 tRNA-synt_1_2, 1 hit
PRINTSiPR00985 TRNASYNTHLEU
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00396 leuS_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B8EQP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFERYNARE TEPKWRDKWE RSATFKTDND DPRPKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYTMG DVIARYKRAR GFSVLHPMGW DAFGMPAENA AMQNKSHPAA
110 120 130 140 150
WTYANIEAMR AQLKSMGLSL DWSREIATCD PAYYRHQQKM FLDFLAAGLV
160 170 180 190 200
DRKKSKVNWD PVDHTVLANE QVIDGRGWRS GALVEQRELT QWFFKITDYA
210 220 230 240 250
EDLLRSLDGL TRWPEKVRLM QANWIGRSEG LLVRFALEPT LSAPATEIEV
260 270 280 290 300
YTTRPDTLFG AKFLAIAADH PIAADCAKAD PALAAFIAEC RLRGTSVAAI
310 320 330 340 350
ETAEKKGVDT GLRVRHPFDE NWLLPVYVAN FVLMDYGTGA IFGCPAHDQR
360 370 380 390 400
DLDFANAYGL GATPVILPPD ADPKTFVIAD KAYDGDGLLF HSRFLDGMTV
410 420 430 440 450
EAAQEEVARR LEEHPLGNRP QAKRQVNFRL RDWGISRQRY WGCPIPIIHC
460 470 480 490 500
PEHGAVPVPA KDLPVELPPD VSFDEPGNPL DRHPTWKHVN CPICAAPSLR
510 520 530 540 550
ETDTMDTFVD SSWYFARFTQ PWMEDAPTDP SAIAKWLPVD QYIGGVEHAI
560 570 580 590 600
LHLLYARFFT RAMQATGHAG EIREPFAGLF TQGMVVHETY RSEAGDWMFP
610 620 630 640 650
ADVRIEIGPA GRRAFDLASG AEVSIGGIEK MSKSKRNTVD PDEIIGTFGA
660 670 680 690 700
DTARWFVLSD SPPERDVIWT EEGVQGAAKF VQRLWRLIGE LIFLSGADGA
710 720 730 740 750
PKPQSISPQA AAIRRAAHQS LIRLEEDLDR LRFNRAVAQA HDLANKLGAA
760 770 780 790 800
VGDIDSVEIA PDLRFAFREA AEILTLMIAP MMPHLAEECW ARLGHKGLAA
810 820 830 840 850
EAPWPQADHS LVVEETIDLP VQVNGKKRGD LIIDRAAGRE AIEAAALALE
860 870
PVKRALEGRP VKKIIIVPQR IVNVVA
Length:876
Mass (Da):97,986
Last modified:March 3, 2009 - v1
Checksum:iD68C834EEF4747A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA Translation: ACK49315.1
RefSeqiWP_012589385.1, NC_011666.1

Genome annotation databases

EnsemblBacteriaiACK49315; ACK49315; Msil_0336
KEGGimsl:Msil_0336

Similar proteinsi

Entry informationi

Entry nameiSYL_METSB
AccessioniPrimary (citable) accession number: B8EQP4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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