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B8EQP4

- SYL_METSB

UniProt

B8EQP4 - SYL_METSB

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei633 – 6331ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSIL395965:GCND-342-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:Msil_0336
    OrganismiMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
    Taxonomic identifieri395965 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
    ProteomesiUP000002257: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 876876Leucine--tRNA ligasePRO_1000199214Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi395965.Msil_0336.

    Structurei

    3D structure databases

    ProteinModelPortaliB8EQP4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi43 – 5311"HIGH" regionAdd
    BLAST
    Motifi630 – 6345"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8EQP4-1 [UniParc]FASTAAdd to Basket

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    MDFERYNARE TEPKWRDKWE RSATFKTDND DPRPKYYVLE MFPYPSGRIH    50
    MGHVRNYTMG DVIARYKRAR GFSVLHPMGW DAFGMPAENA AMQNKSHPAA 100
    WTYANIEAMR AQLKSMGLSL DWSREIATCD PAYYRHQQKM FLDFLAAGLV 150
    DRKKSKVNWD PVDHTVLANE QVIDGRGWRS GALVEQRELT QWFFKITDYA 200
    EDLLRSLDGL TRWPEKVRLM QANWIGRSEG LLVRFALEPT LSAPATEIEV 250
    YTTRPDTLFG AKFLAIAADH PIAADCAKAD PALAAFIAEC RLRGTSVAAI 300
    ETAEKKGVDT GLRVRHPFDE NWLLPVYVAN FVLMDYGTGA IFGCPAHDQR 350
    DLDFANAYGL GATPVILPPD ADPKTFVIAD KAYDGDGLLF HSRFLDGMTV 400
    EAAQEEVARR LEEHPLGNRP QAKRQVNFRL RDWGISRQRY WGCPIPIIHC 450
    PEHGAVPVPA KDLPVELPPD VSFDEPGNPL DRHPTWKHVN CPICAAPSLR 500
    ETDTMDTFVD SSWYFARFTQ PWMEDAPTDP SAIAKWLPVD QYIGGVEHAI 550
    LHLLYARFFT RAMQATGHAG EIREPFAGLF TQGMVVHETY RSEAGDWMFP 600
    ADVRIEIGPA GRRAFDLASG AEVSIGGIEK MSKSKRNTVD PDEIIGTFGA 650
    DTARWFVLSD SPPERDVIWT EEGVQGAAKF VQRLWRLIGE LIFLSGADGA 700
    PKPQSISPQA AAIRRAAHQS LIRLEEDLDR LRFNRAVAQA HDLANKLGAA 750
    VGDIDSVEIA PDLRFAFREA AEILTLMIAP MMPHLAEECW ARLGHKGLAA 800
    EAPWPQADHS LVVEETIDLP VQVNGKKRGD LIIDRAAGRE AIEAAALALE 850
    PVKRALEGRP VKKIIIVPQR IVNVVA 876
    Length:876
    Mass (Da):97,986
    Last modified:March 3, 2009 - v1
    Checksum:iD68C834EEF4747A5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001280 Genomic DNA. Translation: ACK49315.1.
    RefSeqiYP_002360677.1. NC_011666.1.

    Genome annotation databases

    EnsemblBacteriaiACK49315; ACK49315; Msil_0336.
    GeneIDi7091555.
    KEGGimsl:Msil_0336.
    PATRICi22595947. VBIMetSil55537_0369.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001280 Genomic DNA. Translation: ACK49315.1 .
    RefSeqi YP_002360677.1. NC_011666.1.

    3D structure databases

    ProteinModelPortali B8EQP4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395965.Msil_0336.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK49315 ; ACK49315 ; Msil_0336 .
    GeneIDi 7091555.
    KEGGi msl:Msil_0336.
    PATRICi 22595947. VBIMetSil55537_0369.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci MSIL395965:GCND-342-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
      Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
      J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BL2 / DSM 15510 / NCIMB 13906.

    Entry informationi

    Entry nameiSYL_METSB
    AccessioniPrimary (citable) accession number: B8EQP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3