B8EQB9 (ISPH_METSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Msil_3225

Organism

Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP]

Taxonomic identifier

395965 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000124289

Sequences

	Sequence		Length	Mass (Da)
	B8EQB9 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 772FBDB5E2359C23	FASTA	319	34,934
	10 20 30 40 50 60 MTVKPKLNVL LCAPRGFCAG VVRAIDAVEE ALRIYGAPVY VRHEIVHNKY VVETLKSKGA 70 80 90 100 110 120 VFVEELDEVP DTSQPVIFSA HGVPKSIPVE AATRNIFAID ATCPLVTKVH REAELHHKRG 130 140 150 160 170 180 RQVLLVGHAG HPEVVGTIGQ LPAGSILLVQ TIEDIASLQV EDEHNLSYVT QTTLSVDDTR 190 200 210 220 230 240 SMVEALTRRF PDIVGPHRED ICYATTNRQE AVKQVAPIVD ALIVVGSSNS SNSQRLKEVA 250 260 270 280 290 300 ERSGCKLARL VLRAEDVEWE LFANISSLAI TAGASAPEIL VEEIMDAFAE RFDLHVEEVS 310 TANEGVFFPL PRELRRATV

References

[1]

"Complete sequence of Methylocella silvestris BL2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001280 Genomic DNA. Translation: ACK52132.1.
RefSeq	YP_002363494.1. NC_011666.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8EQB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7090640.
GenomeReviews	Gene locus Msil_3225 in contig CP001280_GR.
KEGG	msl:Msil_3225.
PATRIC	22602220. VBIMetSil55537_3460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335228.
OMA	TVSLIET.
ProtClustDB	PRK01045.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_METSB

Accession

Primary (citable) accession number: B8EQB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program