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B8EQB6

- B8EQB6_METSB

UniProt

B8EQB6 - B8EQB6_METSB

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Protein

DNA replication and repair protein RecF

Gene

recF

Organism
Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 578ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. single-stranded DNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. DNA replication Source: UniProtKB-HAMAP
  3. SOS response Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, DNA replicationUniRule annotation, SOS responseUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, DNA-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSIL395965:GCND-3274-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication and repair protein RecFUniRule annotation
Gene namesi
Name:recFUniRule annotation
Ordered Locus Names:Msil_3222Imported
OrganismiMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)Imported
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
ProteomesiUP000002257: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi395965.Msil_3222.

Family & Domainsi

Sequence similaritiesi

Belongs to the RecF family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1195.
HOGENOMiHOG000269558.
KOiK03629.
OMAiYDEICAL.
OrthoDBiEOG6HTP3X.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00365. RecF.
InterProiIPR001238. DNA-binding_RecF.
IPR018078. DNA-binding_RecF_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00611. recf. 1 hit.
PROSITEiPS00617. RECF_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8EQB6-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MQTEALATES SPAPPAKQAR RGVRRLTLAD FRSYASLDME ILAQTVVLTG
60 70 80 90 100
DNGAGKTNVL EALSLLTPGR GLRRAELSDC ARNFGGGGFA VSIEIDAEGG
110 120 130 140 150
RLQLGTGVEP NGGAALARKF RIDREPAPSI RAFCDHIRVV WLTPAMDGLF
160 170 180 190 200
VGSPGDRRRF LDRLVLSLDA DHGARVNALE RALRSRNRLL EERGAGNERL
210 220 230 240 250
WLDAVEREVA ELAIAVAAAR FETVSKLAAL IAQAGPETEG GFPLAELSLD
260 270 280 290 300
GDIDRLIETR PALEAEDEYR KILRDNRGRD AAAGRTLIGP QSSDLAVRHA
310 320 330 340 350
RKQAAASHSS TGEQKALLVG LILAQARLIK AMSGLAPLVL LDEIAAHFDP
360 370 380 390
KRRAALYELL GALGAQVWLT GADPSAFAEL EGKAQMLQVT PGAIRELH
Length:398
Mass (Da):42,643
Last modified:March 3, 2009 - v1
Checksum:i66E5526AD4C1B8A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001280 Genomic DNA. Translation: ACK52129.1.
RefSeqiYP_002363491.1. NC_011666.1.

Genome annotation databases

EnsemblBacteriaiACK52129; ACK52129; Msil_3222.
GeneIDi7090637.
KEGGimsl:Msil_3222.
PATRICi22602214. VBIMetSil55537_3457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001280 Genomic DNA. Translation: ACK52129.1 .
RefSeqi YP_002363491.1. NC_011666.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395965.Msil_3222.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK52129 ; ACK52129 ; Msil_3222 .
GeneIDi 7090637.
KEGGi msl:Msil_3222.
PATRICi 22602214. VBIMetSil55537_3457.

Phylogenomic databases

eggNOGi COG1195.
HOGENOMi HOG000269558.
KOi K03629.
OMAi YDEICAL.
OrthoDBi EOG6HTP3X.

Enzyme and pathway databases

BioCyci MSIL395965:GCND-3274-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_00365. RecF.
InterProi IPR001238. DNA-binding_RecF.
IPR018078. DNA-binding_RecF_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR00611. recf. 1 hit.
PROSITEi PS00617. RECF_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
    Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
    J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BL2 / DSM 15510 / NCIMB 13906Imported.

Entry informationi

Entry nameiB8EQB6_METSB
AccessioniPrimary (citable) accession number: B8EQB6
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3