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B8EQB6

- B8EQB6_METSB

UniProt

B8EQB6 - B8EQB6_METSB

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Protein
DNA replication and repair protein RecF
Gene
recF, Msil_3222
Organism
Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Status
Unreviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP By similarity.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 578ATP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. single-stranded DNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. DNA replication Source: UniProtKB-HAMAP
  3. SOS response Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, DNA replicationUniRule annotationSAAS annotations, SOS responseUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, DNA-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSIL395965:GCND-3274-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication and repair protein RecFUniRule annotation
Gene namesi
Name:recFUniRule annotation
Ordered Locus Names:Msil_3222Imported
OrganismiMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)Imported
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
ProteomesiUP000002257: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Protein-protein interaction databases

STRINGi395965.Msil_3222.

Family & Domainsi

Sequence similaritiesi

Belongs to the RecF family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1195.
HOGENOMiHOG000269558.
KOiK03629.
OMAiYDEICAL.
OrthoDBiEOG6HTP3X.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00365. RecF.
InterProiIPR001238. DNA-binding_RecF.
IPR018078. DNA-binding_RecF_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00611. recf. 1 hit.
PROSITEiPS00617. RECF_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8EQB6-1 [UniParc]FASTAAdd to Basket

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MQTEALATES SPAPPAKQAR RGVRRLTLAD FRSYASLDME ILAQTVVLTG    50
DNGAGKTNVL EALSLLTPGR GLRRAELSDC ARNFGGGGFA VSIEIDAEGG 100
RLQLGTGVEP NGGAALARKF RIDREPAPSI RAFCDHIRVV WLTPAMDGLF 150
VGSPGDRRRF LDRLVLSLDA DHGARVNALE RALRSRNRLL EERGAGNERL 200
WLDAVEREVA ELAIAVAAAR FETVSKLAAL IAQAGPETEG GFPLAELSLD 250
GDIDRLIETR PALEAEDEYR KILRDNRGRD AAAGRTLIGP QSSDLAVRHA 300
RKQAAASHSS TGEQKALLVG LILAQARLIK AMSGLAPLVL LDEIAAHFDP 350
KRRAALYELL GALGAQVWLT GADPSAFAEL EGKAQMLQVT PGAIRELH 398
Length:398
Mass (Da):42,643
Last modified:March 3, 2009 - v1
Checksum:i66E5526AD4C1B8A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001280 Genomic DNA. Translation: ACK52129.1.
RefSeqiWP_012592198.1. NC_011666.1.
YP_002363491.1. NC_011666.1.

Genome annotation databases

EnsemblBacteriaiACK52129; ACK52129; Msil_3222.
GeneIDi7090637.
KEGGimsl:Msil_3222.
PATRICi22602214. VBIMetSil55537_3457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001280 Genomic DNA. Translation: ACK52129.1 .
RefSeqi WP_012592198.1. NC_011666.1.
YP_002363491.1. NC_011666.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395965.Msil_3222.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK52129 ; ACK52129 ; Msil_3222 .
GeneIDi 7090637.
KEGGi msl:Msil_3222.
PATRICi 22602214. VBIMetSil55537_3457.

Phylogenomic databases

eggNOGi COG1195.
HOGENOMi HOG000269558.
KOi K03629.
OMAi YDEICAL.
OrthoDBi EOG6HTP3X.

Enzyme and pathway databases

BioCyci MSIL395965:GCND-3274-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_00365. RecF.
InterProi IPR001238. DNA-binding_RecF.
IPR018078. DNA-binding_RecF_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR00611. recf. 1 hit.
PROSITEi PS00617. RECF_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
    Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
    J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BL2 / DSM 15510 / NCIMB 13906.

Entry informationi

Entry nameiB8EQB6_METSB
AccessioniPrimary (citable) accession number: B8EQB6
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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