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B8EPW8 (B8EPW8_METSB) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840
Short name=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase HAMAP MF_01840
EC=1.14.13.81 HAMAP MF_01840
Gene names
Name:acsF HAMAP MF_01840
Ordered Locus Names:Msil_2032
OrganismMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP] EMBL ACK50972.1
Taxonomic identifier395965 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity. HAMAP MF_01840

Catalytic activity

131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840

131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840

Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840

Cofactor

Iron By similarity. HAMAP MF_01840

Pathway

Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent). HAMAP MF_01840

Sequence similarities

Belongs to the AcsF family. HAMAP MF_01840

Sequences

Sequence LengthMass (Da)Tools
B8EPW8 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 23891BE385C42BE9

FASTA35340,645
        10         20         30         40         50         60 
MTADRKSAPN ETTAMATADT VLSPRFYTTD FAEIDRIDVG PVRTEWDALI DEMARDPNQG 

        70         80         90        100        110        120 
HFVRTDEFDT DLSHLPEALR KEFVDFLVSS VTAEFSGCVL YAELRKRASN PDIRALFGYM 

       130        140        150        160        170        180 
SRDEARHAGF INATLKDLKI GVDLGFLTKA KKYTFFKPKF IFYATYLSEK IGYARYITIF 

       190        200        210        220        230        240 
RQLERYPERR IHPIFKWFDK WCNDEFRHGE AFALLMRANP KLLSGMNVYW VKFFLIAVFA 

       250        260        270        280        290        300 
TMYVRDHARP EFHKALGLTP DEYDFAVFRI TSEISRQTFP LALDLDNPAF HAGLDRLRRI 

       310        320        330        340        350 
GEAMTRAEKR GGVLGRMQKL SLTGAAGLAF VRLYLVPAIR NDMPANIRLS PVW

« Hide

References

[1]"Complete sequence of Methylocella silvestris BL2."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. expand/collapse author list , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001280 Genomic DNA. Translation: ACK50972.1.
RefSeqYP_002362334.1. NC_011666.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB8EPW8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7094230.
GenomeReviewsGene locus Msil_2032 in contig CP001280_GR.
KEGGmsl:Msil_2032.
PATRIC22599665. VBIMetSil55537_2200.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG284843.
OMAHGDFFDA.
ProtClustDBPRK13654.

Family and domain databases

HAMAPMF_01840. AcsF.
[Tree]
InterProIPR008434. AcsF.
IPR012347. Ferritin-rel.
IPR009078. Ferritin/RR-like.
IPR003251. Rubrerythrin.
[Graphical view]
Gene3DG3DSA:1.20.1260.10. Ferritin_rel. 1 hit.
KOK04035.
PfamPF02915. Rubrerythrin. 1 hit.
[Graphical view]
SUPFAMSSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMsTIGR02029. AcsF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB8EPW8_METSB
AccessionPrimary (citable) accession number: B8EPW8
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info