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Protein

Urease subunit alpha

Gene

ureC

Organism
Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit gamma (ureA), Urease subunit beta (ureB)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi136 – 1361Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi138 – 1381Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi219 – 2191Nickel 1; via carbamate groupUniRule annotation
Metal bindingi219 – 2191Nickel 2; via carbamate groupUniRule annotation
Binding sitei221 – 2211SubstrateUniRule annotation
Metal bindingi248 – 2481Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi274 – 2741Nickel 2; via tele nitrogenUniRule annotation
Active sitei322 – 3221Proton donorUniRule annotation
Metal bindingi362 – 3621Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciMSIL395965:GCND-2047-MONOMER.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:Msil_2011
OrganismiMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
Proteomesi
  • UP000002257 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Urease subunit alphaPRO_1000188882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei219 – 2191N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi395965.Msil_2011.

Structurei

3D structure databases

ProteinModelPortaliB8EPU9.
SMRiB8EPU9. Positions 5-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 570440UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8EPU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVTMSRRAY AGMFGPTTGD KIRLADTELL IEVERDFTIY GEEVKFGGGK
60 70 80 90 100
VIRDGMGQSQ RSRAEGAVDT LITNVVILDH TGVVKADVGL KDGRIAAIGK
110 120 130 140 150
GGNPDIQPGV TITIGPGTEI IAGEGKILTA GGVDTHIHFI APQQIEEALC
160 170 180 190 200
SGVTTMLGGG SGPAEGTKAT TCTSGPWHMM RMMQAAESLP MNIGFFGKGN
210 220 230 240 250
ASKPAALIEM VEAGACGLKL HEDWGTTPAA IDCCLSVCDA LDVQATLHSD
260 270 280 290 300
TLNESGFVET TIAAFKGRTI HAFHTEGAGG GHAPDIIKVV GEQNVLPSST
310 320 330 340 350
NPTRPFTRNT LDEHLDMLMV CHHLDPHIAE DIAFAESRIR KETIAAEDIL
360 370 380 390 400
HDLGALSMFS SDSQAMGRVG EVVIRCWQTA DKMKRQRGAL PEDISGNDNF
410 420 430 440 450
RARRYIAKYT INPAIAHGIS HVVGSVEPGK LADLVLWSPA FFGVKPDLVL
460 470 480 490 500
KGGSIAYALM GDANASIPTP QPVHYRPMFA SFGRSLIESS LIFVSAVSIE
510 520 530 540 550
RGVGARYGLS RPLEAVRGTR GSISKKSMIL NDATPVMEVD PETYEVRADG
560 570
ELLTCEPADV LPMAQRYFLF
Length:570
Mass (Da):60,826
Last modified:March 3, 2009 - v1
Checksum:i3A1C2214514C1257
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA. Translation: ACK50953.1.
RefSeqiWP_012591023.1. NC_011666.1.

Genome annotation databases

EnsemblBacteriaiACK50953; ACK50953; Msil_2011.
KEGGimsl:Msil_2011.
PATRICi22599621. VBIMetSil55537_2177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA. Translation: ACK50953.1.
RefSeqiWP_012591023.1. NC_011666.1.

3D structure databases

ProteinModelPortaliB8EPU9.
SMRiB8EPU9. Positions 5-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395965.Msil_2011.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK50953; ACK50953; Msil_2011.
KEGGimsl:Msil_2011.
PATRICi22599621. VBIMetSil55537_2177.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciMSIL395965:GCND-2047-MONOMER.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
    Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
    J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BL2 / DSM 15510 / NCIMB 13906.

Entry informationi

Entry nameiURE1_METSB
AccessioniPrimary (citable) accession number: B8EPU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 11, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.