B8EPJ0 (ACSA_METSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	Msil_1226

Organism

Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP]

Taxonomic identifier

395965 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000164051
Sites
	Active site	515	1	By similarity
Amino acid modifications
	Modified residue	607	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8EPJ0 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 79DAA68C78D3C927	FASTA	645	70,912
	10 20 30 40 50 60 MSDKVYPVTA EWSKSAYVDA AKYKSMYESS VSEPDKFWGE HGKRIDWVKP YTKVKNTSFD 70 80 90 100 110 120 PHNVSIKWFE DGVTNVSTNC IDRHLATLGD QTAIIWEGDD PNESKHITYK QLHEEVCRLA 130 140 150 160 170 180 NVLKSYGISK GDTVTIYLPM IPEAAYAMLA CARIGAIHSI VFGGFSADSL GGRIEGCKSK 190 200 210 220 230 240 LIITADEGYR GGRKVPLKVN ADAAIKKVDG IVETMIVVRR TGGKVAWTEG RDVWYDEALA 250 260 270 280 290 300 KASPDCPAAE MNAEDPLFIL FTSGSTGAPK GVVHSTGGYL VWASMTHQYV FDYRPGEVYW 310 320 330 340 350 360 CTADVGWVTG HSYIVYGPLA NGATTLMFEG VPSYPTISRF WDVVDKHQVN IFYTAPTAIR 370 380 390 400 410 420 SLMGAGDGPV KATSRKTLRV LGSVGEPINP EAWEWYYRVV GEERCPIVDT WWQTETGGIL 430 440 450 460 470 480 ISPLPGATAL KPGSATQPFF GVQPQVVDAA GEVLEGPCSG NLVIADSWPA QMRTLFNDHE 490 500 510 520 530 540 RFVQAYFSAY PGKYFTGDGC RRDEDGYYWI TGRVDDVINV SGHRLGTAEV ESSLVAHIKV 550 560 570 580 590 600 AEAAVVGYPH DLKGQGIYAY VTLMTGIEPS EELRKELVSW VRKDIGPIAS PDIVHFAPGL 610 620 630 640 PKTRSGKIMR RILRKIAEKE FSALGDTSTL ADPSVVDDLI RERAN

References

[1]

"Complete sequence of Methylocella silvestris BL2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001280 Genomic DNA. Translation: ACK50195.1.
RefSeq	YP_002361557.1. NC_011666.1.
3D structure databases
ProteinModelPortal	B8EPJ0.
ModBase	Search...
Protein-protein interaction databases
STRING	B8EPJ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7092299.
GenomeReviews	Gene locus Msil_1226 in contig CP001280_GR.
KEGG	msl:Msil_1226.
PATRIC	22597937. VBIMetSil55537_1347.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	LIQWAGG.
ProtClustDB	PRK00174.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_METSB

Accession

Primary (citable) accession number: B8EPJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program