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B8EPH9 (GCSPA_METSB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable glycine dehydrogenase (decarboxylating) subunit 1

EC=1.4.4.2
Alternative name(s):
Glycine cleavage system P-protein subunit 1
Glycine decarboxylase subunit 1
Glycine dehydrogenase (aminomethyl-transferring) subunit 1
Gene names
Name:gcvPA
Ordered Locus Names:Msil_1215
OrganismMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP]
Taxonomic identifier395965 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP-Rule MF_00712

Catalytic activity

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2. HAMAP-Rule MF_00712

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits By similarity.

Sequence similarities

Belongs to the GcvP family. N-terminal subunit subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Probable glycine dehydrogenase (decarboxylating) subunit 1 HAMAP-Rule MF_00712
PRO_1000147990

Sequences

Sequence LengthMass (Da)Tools
B8EPH9 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 16B94903586E6685

FASTA44647,990
        10         20         30         40         50         60 
MRYLPLNSED RSEMLARIGV DSIDALFSSI PEASRLSRDL DLPLRRSEME TQRLLSGMAA 

        70         80         90        100        110        120 
RNTPASSAPF FIGAGAYKHH IPASVDHLIQ RSEFLTSYTP YQPEISQGTL QAIFEFQTQV 

       130        140        150        160        170        180 
AALTAMDVAN ASMYDGSTAT MEAVLMAHRV TKRRKAVLSG GLHPHYADVV RTVSKMTDND 

       190        200        210        220        230        240 
ALSLPPDVFA AEDLAAEIDE ETSCVVVQTP DAFGNLRDLS PLAAACKEHG ALLIAVFTEA 

       250        260        270        280        290        300 
VSLGLVKPPG AMGADIVVGE GQSIGNGLNF GGPYVGLFAT RQKYLRQMPG RLCGETVDAD 

       310        320        330        340        350        360 
GRRGFVLTLS TREQHIKRDK ATSSICTNAG LCCLAFTMHL TLLGEAGLRQ LARVNHANAV 

       370        380        390        400        410        420 
DLAGRFAQIY GVEVLNRSFF NEFTLRVPGP AAEIVEHLAQ KGILGGVPAS RLWPGEDLDD 

       430        440 
LMIVASSEIN TDEDRAAFAS ALRGIA 

« Hide

References

[1]"Complete sequence of Methylocella silvestris BL2."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. expand/collapse author list , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001280 Genomic DNA. Translation: ACK50184.1.
RefSeqYP_002361546.1. NC_011666.1.

3D structure databases

ProteinModelPortalB8EPH9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395965.Msil_1215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK50184; ACK50184; Msil_1215.
GeneID7092288.
KEGGmsl:Msil_1215.
PATRIC22597915. VBIMetSil55537_1336.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0403.
HOGENOMHOG000132025.
KOK00282.
OMASMYDGST.
OrthoDBEOG6XWV3B.
ProtClustDBPRK00451.

Enzyme and pathway databases

BioCycMSIL395965:GCND-1239-MONOMER.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
HAMAPMF_00712. GcvPA.
InterProIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR023010. GDC_P_su1.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11773. PTHR11773. 1 hit.
PfamPF02347. GDC-P. 1 hit.
[Graphical view]
PIRSFPIRSF006815. GcvPA. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGCSPA_METSB
AccessionPrimary (citable) accession number: B8EPH9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families