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B8EPF9 (B8EPF9_METSB) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338

Short name=RuBisCO large subunit HAMAP-Rule MF_01338
EC=4.1.1.39 HAMAP-Rule MF_01338
Gene names
Name:cbbL HAMAP-Rule MF_01338
Ordered Locus Names:Msil_1195 EMBL ACK50164.1
OrganismMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP] EMBL ACK50164.1
Taxonomic identifier395965 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains By similarity. HAMAP-Rule MF_01338

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity. HAMAP-Rule MF_01338

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily. HAMAP-Rule MF_01338

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1761Proton acceptor By similarity HAMAP-Rule MF_01338
Active site2941Proton acceptor By similarity HAMAP-Rule MF_01338
Metal binding2021Magnesium; via carbamate group By similarity HAMAP-Rule MF_01338
Metal binding2041Magnesium By similarity HAMAP-Rule MF_01338
Metal binding2051Magnesium By similarity HAMAP-Rule MF_01338
Binding site1241Substrate; in homodimeric partner By similarity HAMAP-Rule MF_01338
Binding site1741Substrate By similarity HAMAP-Rule MF_01338
Binding site1781Substrate By similarity HAMAP-Rule MF_01338
Binding site2951Substrate By similarity HAMAP-Rule MF_01338
Binding site3271Substrate By similarity HAMAP-Rule MF_01338
Binding site3791Substrate By similarity HAMAP-Rule MF_01338
Site3341Transition state stabilizer By similarity HAMAP-Rule MF_01338

Amino acid modifications

Modified residue2021N6-carboxylysine By similarity HAMAP-Rule MF_01338

Sequences

Sequence LengthMass (Da)Tools
B8EPF9 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 07A90129067ABB20

FASTA48453,508
        10         20         30         40         50         60 
MTASPPAQPR SRYSAGVMEY REMGYWQPDY VPGETDIIAC FRVTPQEGVD PIEASAAVAG 

        70         80         90        100        110        120 
ESSTATWTVV WTDRLTASEK YRAKCYRVDP VPNAPGSWFA FIAYDLDLFE PGSISNLAAS 

       130        140        150        160        170        180 
IIGNVFGFKP LRALRLEDMR FPVAYVKTFQ GPATGIVVER ERLDKFGRPL LGATVKPKLG 

       190        200        210        220        230        240 
LSGRNYGRVV YEALKGGLDF TKDDENINSQ PFMHWRDRFL YCMEAVNRAE AATGEIKGTY 

       250        260        270        280        290        300 
LNVTAATMED MYERAEFAKE LGSVIIMIDL VIGYTAIQSM AKWARRNDMI LHLHRAGHST 

       310        320        330        340        350        360 
YTRQKIHGVS FRVIAKWMRL AGVDHIHAGT VVGKLEGDPN TTRGYYDICR EDYNPQQLEH 

       370        380        390        400        410        420 
GIFFDQHWAS LNKLMPVASG GIHAGQMHQL LALLGEDVVL QFGGGTIGHP MGIAAGATAN 

       430        440        450        460        470        480 
RVALEAMIFA RNCGRDTLAE GQTILEDAAR GCTPLRQALD VWKDVSFDYT STDSPDFIPT 


ATPA 

« Hide

References

[1]"Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001280 Genomic DNA. Translation: ACK50164.1.
RefSeqYP_002361526.1. NC_011666.1.

3D structure databases

ProteinModelPortalB8EPF9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395965.Msil_1195.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK50164; ACK50164; Msil_1195.
GeneID7092268.
KEGGmsl:Msil_1195.
PATRIC22597875. VBIMetSil55537_1316.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1850.
HOGENOMHOG000230831.
KOK01601.
OMAFTQDWAS.
OrthoDBEOG6ZKXMS.

Enzyme and pathway databases

BioCycMSIL395965:GCND-1219-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB8EPF9_METSB
AccessionPrimary (citable) accession number: B8EPF9
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)