B8ENQ7 (B8ENQ7_METSB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase HAMAP MF_01374
EC=3.1.2.6 HAMAP MF_01374

Alternative name(s):
Glyoxalase II HAMAP MF_01374

Gene names

Name:	gloB HAMAP MF_01374
Ordered Locus Names:	Msil_1898

Organism

Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP] EMBL ACK50843.1

Taxonomic identifier

395965 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP MF_01374

Ontologies

Keywords
Ligand	Metal-binding HAMAP MF_01374 Zinc HAMAP MF_01374
Molecular function	Hydrolase HAMAP MF_01374 EMBL ACK50843.1
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	56	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	58	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	60	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	61	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	115	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	134	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	134	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	172	1	Zinc 2 By similarity HAMAP MF_01374

Sequences

	Sequence		Length	Mass (Da)
	B8ENQ7 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: CE84C7831EBAE13D	FASTA	256	27,222
	10 20 30 40 50 60 MAPHIHQFIC LSDNFGVLVH DPETRATCAV DAPDGAPILA ALAAREWELT DILLTHHHAD 70 80 90 100 110 120 HVQGVGALKA AFPKARVVGP LKEAEKIGGL DLKVGEGDLV MVGSLAARVI AVPGHTSGHV 130 140 150 160 170 180 AYWFEEEDLA FVGDTLFAMG CGRAFEEPPD VLFHSLSKLA ALPDETQVYC GHEYTLANAK 190 200 210 220 230 240 FALSVEPGNT LLKERAGEVA ALRAAGKFTL PTTISIELAT NPFLRAEEPE LQAAVGMPGA 250 DPAAVFAVLR ERKNHA

References

[1]

"Complete sequence of Methylocella silvestris BL2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001280 Genomic DNA. Translation: ACK50843.1.
RefSeq	YP_002362205.1. NC_011666.1.
3D structure databases
ProteinModelPortal	B8ENQ7.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ENQ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7094014.
GenomeReviews	Gene locus Msil_1898 in contig CP001280_GR.
KEGG	msl:Msil_1898.
PATRIC	22599389. VBIMetSil55537_2063.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	IGCGRVF.
ProtClustDB	CLSK2405212.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8ENQ7_METSB

Accession

Primary (citable) accession number: B8ENQ7

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)