B8ENF6 (B8ENF6_METSB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	Msil_1118

Organism

Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP] EMBL ACK50087.1

Taxonomic identifier

395965 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 EMBL ACK50087.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	97 – 107	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	12	1	By similarity HAMAP MF_00061
	Active site	139	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	B8ENF6 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 4F4DF0D8B3832234	FASTA	291	30,574
	10 20 30 40 50 60 MPLPLIERAP AKINLTLHIL GRRADGWHEL ESLVVFSRTG DQLSLIEGEP LSLTIEGPTA 70 80 90 100 110 120 PAAGEVADNL VLRAARHFAA AIPDAHLGAF HLVKRLPVAA GIGGGSSDAA AALRLLARAN 130 140 150 160 170 180 GVPPDDERLI AAARKTGADV PVCLAARGRI MRGFGDELGP VLKLPPLVAL IVNPNEPLET 190 200 210 220 230 240 RRVFERMDIA PGARTDFGGH PKLASGMGPE ELIAALAKGR NDMEPAACRL APIIGDVLSI 250 260 270 280 290 LGSAPGAKLA RMSGSGATCF ALFKDCKSAA RARKAILRAH PRWWVKSCVL N

References

[1]

"Complete sequence of Methylocella silvestris BL2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001280 Genomic DNA. Translation: ACK50087.1.
RefSeq	YP_002361449.1. NC_011666.1.
3D structure databases
ProteinModelPortal	B8ENF6.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ENF6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7093880.
GenomeReviews	Gene locus Msil_1118 in contig CP001280_GR.
KEGG	msl:Msil_1118.
PATRIC	22597707. VBIMetSil55537_1233.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	KINLHLG.
ProtClustDB	PRK14608.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8ENF6_METSB

Accession

Primary (citable) accession number: B8ENF6

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)