B8EN55 (SYD_METSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Msil_0209

Organism

Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP]

Taxonomic identifier

395965 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000199000

Sequences

	Sequence		Length	Mass (Da)
	B8EN55 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: E8EB77C98FD9B337	FASTA	623	70,061
	10 20 30 40 50 60 MHRYRSHTCG DLRETDALQP TRLSGWCHRI RDHGGVLFID LRDHYGITQC VVDPDSGAFP 70 80 90 100 110 120 LAEKLRSEWV VRIDGLVRQR PAGTENIEMP TGLIEVYVTE IEVLGAAAEL PLPVFGDQNY 130 140 150 160 170 180 PEDMRLRYRF LDLRREKLHN NIMLRGRVID SLRARMKGQG FFEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRLHPGK FYALPQAPQQ FKQLLMTAGF DRYFQIAPCF RDEDLRADRS LEFYQLDIEM 250 260 270 280 290 300 SFVTQEDIFA TVEPVLRGVF EEFGEGFAVT PEFPRIPYAE TMLKYGTDKP DLRNPLVIVD 310 320 330 340 350 360 VTEEFSREDV SFNAFKNVIK QGGVVRAIPA PGAGAQPRSF FDKLNDWARG EGAAGLGYVI 370 380 390 400 410 420 FEGDAGALVG KGPIAKFLPA DVQQAIAAKA GLKSGDAVFF ACDRPDRAAK LAGAARLRIG 430 440 450 460 470 480 SELKLSKTGV FELCWIVDFP MYEWNEDEKR IDFSHNPFSM PNCNLEQFLA LETGVREEIV 490 500 510 520 530 540 GIDRGERNRD LNESNELRKR ILEITAFQYD VVCNGFELSS GAIRNHRPDI MRKAFALAGY 550 560 570 580 590 600 DETVLEQKFG GMYRAFHYGA PPHGGIAPGV DRIVMLLAGE ENLREIVAFP MNQRGEDLLL 610 620 GAPSNVTAKQ LRELSIKLNL PEK

References

[1]

"Complete sequence of Methylocella silvestris BL2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001280 Genomic DNA. Translation: ACK49190.1.
RefSeq	YP_002360552.1. NC_011666.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8EN55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7090526.
GenomeReviews	Gene locus Msil_0209 in contig CP001280_GR.
KEGG	msl:Msil_0209.
PATRIC	22595663. VBIMetSil55537_0229.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_METSB

Accession

Primary (citable) accession number: B8EN55

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program