B8EMX8 (B8EMX8_METSB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1 HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022

Alternative name(s):
Glutamyl-tRNA synthetase 1 HAMAP MF_00022

Gene names

Name:	gltX1 HAMAP MF_00022
Ordered Locus Names:	Msil_0131

Organism

Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP] EMBL ACK49113.1

Taxonomic identifier

395965 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00022
Cellular component	Cytoplasm HAMAP MF_00022
Ligand	ATP-binding HAMAP MF_00022 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00022 EMBL ACK49113.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	10 – 20	11	"HIGH" region By similarity HAMAP MF_00022
	Motif	243 – 247	5	"KMSKS" region By similarity HAMAP MF_00022
Sites
	Binding site	246	1	ATP By similarity HAMAP MF_00022

Sequences

	Sequence		Length	Mass (Da)
	B8EMX8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 16EC4730EAB245C9	FASTA	453	49,761
	10 20 30 40 50 60 MPHPIVRFAP SPTGAIHIGN ARTALLNFLH SKKHHGEFIL RFDDTDAERS KEEYAQAIEV 70 80 90 100 110 120 DLAWLGIVPD RVLRQSQRIA LYESAAKKLR AAGRLYPCYE TAEELERKRR RQQARGQPPV 130 140 150 160 170 180 YDRAALALSA EDKARLEAEG RRPHWRFLLE RRIVRWDDLI RGESHIDSAS LSDPVLIRED 190 200 210 220 230 240 GTFLYTLPSV VDDLDCKVTA IIRGEDHVTN TAVQIELFEA LAGAGAAPGF GHHNLLSSVS 250 260 270 280 290 300 GEGLSKRTGA LSIGGLRDAG LESLAVAAAA VLIGSSLPLH PVVSLAELAS ILDFKQLSRT 310 320 330 340 350 360 QTRFDPAELA ALSARTLHGL GFEAVRDRLG AHEIVGYKAE PFWLAVRGNL SVFLDVVDWW 370 380 390 400 410 420 RVVEGEIAPS APEPGDQAFL KDAAAALPQE PFDEATWSVW INVLKERTGR KGRALFHPLR 430 440 450 LALTGQENGP ELAALLPLIG RFRASARLCG RVA

References

[1]

"Complete sequence of Methylocella silvestris BL2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001280 Genomic DNA. Translation: ACK49113.1.
RefSeq	YP_002360475.1. NC_011666.1.
3D structure databases
ProteinModelPortal	B8EMX8.
ModBase	Search...
Protein-protein interaction databases
STRING	B8EMX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7090447.
GenomeReviews	Gene locus Msil_0131 in contig CP001280_GR.
KEGG	msl:Msil_0131.
PATRIC	22595487. VBIMetSil55537_0142.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	LLYPCYE.
ProtClustDB	PRK12558.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8EMX8_METSB

Accession

Primary (citable) accession number: B8EMX8

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)