B8EM56 (B8EM56_METSB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Arginine biosynthesis bifunctional protein ArgJ HAMAP MF_01106

Gene names

Name:	argJ HAMAP MF_01106
Ordered Locus Names:	Msil_2520

Organism

Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP] EMBL ACK51445.1

Taxonomic identifier

395965 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity. HAMAP MF_01106
Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106 N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity. HAMAP MF_01106
Subcellular location	Cytoplasm By similarity HAMAP MF_01106.
Miscellaneous	Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway By similarity. HAMAP MF_01106
Sequence similarities	Belongs to the ArgJ family. HAMAP MF_01106

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis HAMAP MF_01106
Cellular component	Cytoplasm HAMAP MF_01106
Molecular function	Acyltransferase HAMAP MF_01106 EMBL ACK51445.1 Transferase
PTM	Autocatalytic cleavage HAMAP MF_01106
Technical term	Complete proteome Multifunctional enzyme HAMAP MF_01106
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	193 – 194	2	Cleavage; by autolysis By similarity HAMAP MF_01106

Sequences

	Sequence		Length	Mass (Da)
	B8EM56 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 5D0AB2C8F240F45A	FASTA	412	43,042
	10 20 30 40 50 60 MAALSPLAPQ TYPDLPSIEG VRFAAGAAGI RYKGRTDVML VLMDKGTEVA GVFTKSKCPS 70 80 90 100 110 120 APVDWCRARL PGGKARALIV NSGNANAFTG KAGADAVKLT ATIAAKALGA PKSEIFLAST 130 140 150 160 170 180 GVIGEPLDAS KFDPALEKLA AKAKPDLMFE AAKAIMTTDT YPKVSTATAD LGGVEVTICG 190 200 210 220 230 240 MAKGAGMIAP DMATMLSFIF TDAPIAAAAL QSMLSKGVEG SFNAITVDSD TSTSDTLMLF 250 260 270 280 290 300 ATGAAAKRGA PRIELAADRR LASFKAALDK VLLDLAHQVV RDGEGARHFV EITVTGAAST 310 320 330 340 350 360 AAAKRIALSI ANSPLVKTAI AGEDANWGRI VAAVGKAGEK AERDLLAIWF GEIRVAHRGR 370 380 390 400 410 RDPSYDEAEV SKLMKEKEIK IYVEIGIGTG QATVWTCDLT KEYVEINGDY RS

References

[1]

"Complete sequence of Methylocella silvestris BL2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001280 Genomic DNA. Translation: ACK51445.1.
RefSeq	YP_002362807.1. NC_011666.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8EM56.
Protein family/group databases
MEROPS	T05.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7091071.
GenomeReviews	Gene locus Msil_2520 in contig CP001280_GR.
KEGG	msl:Msil_2520.
PATRIC	22600745. VBIMetSil55537_2732.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284202.
OMA	FPKLATR.
ProtClustDB	CLSK2406008.
Family and domain databases
HAMAP	MF_01106. ArgJ. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
KO	K00620.
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. Arg_biosynth_ArgJ. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56266. Pept_S58_DmpA/Arg_biosyn_ArgJ. 1 hit.
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8EM56_METSB

Accession

Primary (citable) accession number: B8EM56

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)