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Protein

Malate dehydrogenase

Gene

mdh

Organism
Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADUniRule annotation1
Binding sitei83SubstrateUniRule annotation1
Binding sitei89SubstrateUniRule annotation1
Binding sitei96NADUniRule annotation1
Binding sitei121SubstrateUniRule annotation1
Binding sitei152SubstrateUniRule annotation1
Active sitei176Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15NADUniRule annotation6
Nucleotide bindingi119 – 121NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Msil_2501
OrganismiMethylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
Proteomesi
  • UP000002257 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001916511 – 321Malate dehydrogenaseAdd BLAST321

Interactioni

Protein-protein interaction databases

STRINGi395965.Msil_2501.

Structurei

3D structure databases

ProteinModelPortaliB8EM38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.
OrthoDBiPOG091H02N6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B8EM38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSKIALIG AGQIGGTLAH LAALKELGDI VLFDIAEGTP QGKALDLAES
60 70 80 90 100
GPVEGFNASL KGANSYADIA GADVIIVTAG VPRKPGMSRD DLLGINLKVM
110 120 130 140 150
DAVGGGIKQY APDAFVICIT NPLDAMVWAL QKSSGLAPAK IVGMAGVLDS
160 170 180 190 200
ARFRYFLSEE FKVSVEDVTA FVLGGHGDDM VPSLRYSTVA GIPLTDLVKL
210 220 230 240 250
GWTTQERLDA IVERTRKGGG EIVNLLKTGS AFYAPAASAI AMAEAYLKDK
260 270 280 290 300
RRVLPCAAQL SGQYGVDNLY VGVPVVIGAN GVEKIVEVTL DESEKAMFAK
310 320
SVESVRGLVE ACKVINPALA G
Length:321
Mass (Da):33,464
Last modified:March 3, 2009 - v1
Checksum:iF51C3F35AA4FC33F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA. Translation: ACK51427.1.
RefSeqiWP_012591496.1. NC_011666.1.

Genome annotation databases

EnsemblBacteriaiACK51427; ACK51427; Msil_2501.
KEGGimsl:Msil_2501.
PATRICi22600701. VBIMetSil55537_2710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA. Translation: ACK51427.1.
RefSeqiWP_012591496.1. NC_011666.1.

3D structure databases

ProteinModelPortaliB8EM38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395965.Msil_2501.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK51427; ACK51427; Msil_2501.
KEGGimsl:Msil_2501.
PATRICi22600701. VBIMetSil55537_2710.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.
OrthoDBiPOG091H02N6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_METSB
AccessioniPrimary (citable) accession number: B8EM38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.