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B8EM14

- DAPF_METSB

UniProt

B8EM14 - DAPF_METSB

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei17 – 171SubstrateUniRule annotation
    Binding sitei49 – 491SubstrateUniRule annotation
    Binding sitei69 – 691SubstrateUniRule annotation
    Active sitei78 – 781Proton donor/acceptorUniRule annotation
    Binding sitei165 – 1651SubstrateUniRule annotation
    Sitei167 – 1671Important for catalytic activityUniRule annotation
    Binding sitei198 – 1981SubstrateUniRule annotation
    Sitei216 – 2161Important for catalytic activityUniRule annotation
    Active sitei225 – 2251Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciMSIL395965:GCND-1884-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:Msil_1850
    OrganismiMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
    Taxonomic identifieri395965 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
    ProteomesiUP000002257: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290Diaminopimelate epimerasePRO_1000124426Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi78 ↔ 225

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi395965.Msil_1850.

    Structurei

    3D structure databases

    ProteinModelPortaliB8EM14.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni14 – 152Substrate bindingUniRule annotation
    Regioni78 – 803Substrate bindingUniRule annotation
    Regioni216 – 2172Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiDGVWMTG.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8EM14-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPLANRLAV KMNGIGNEIL IVDLRGAGAG VTGPQARAIA RTKGLAFDQL    50
    MVLEDPRSKG CAAFVTIFNA DGSQSAACGN GTRCVAWTLL KDGARDEVLL 100
    ETAAGRLACR RVSELVFSVE MGRPRLKAAE IPLSRDAPDT SAVDLSFGPA 150
    DSPILKNPAV VNMGNPHAIF FVEDIGAYDL AAIGPVLEHD PLFPERANIS 200
    LARIVSPERI ELKVWERGVG LTLACGSAAC AALVAAARRG LTGRRATVAL 250
    PGGELDIFWR ESDDMVVMTG PVAFEFETRL DPAIFADAAA 290
    Length:290
    Mass (Da):30,607
    Last modified:March 3, 2009 - v1
    Checksum:iCBA2F8F9AD691263
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001280 Genomic DNA. Translation: ACK50795.1.
    RefSeqiYP_002362157.1. NC_011666.1.

    Genome annotation databases

    EnsemblBacteriaiACK50795; ACK50795; Msil_1850.
    GeneIDi7094129.
    KEGGimsl:Msil_1850.
    PATRICi22599279. VBIMetSil55537_2008.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001280 Genomic DNA. Translation: ACK50795.1 .
    RefSeqi YP_002362157.1. NC_011666.1.

    3D structure databases

    ProteinModelPortali B8EM14.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395965.Msil_1850.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK50795 ; ACK50795 ; Msil_1850 .
    GeneIDi 7094129.
    KEGGi msl:Msil_1850.
    PATRICi 22599279. VBIMetSil55537_2008.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi DGVWMTG.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci MSIL395965:GCND-1884-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
      Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
      J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BL2 / DSM 15510 / NCIMB 13906.

    Entry informationi

    Entry nameiDAPF_METSB
    AccessioniPrimary (citable) accession number: B8EM14
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3