ID   B8EKC5_METSB            Unreviewed;       919 AA.
AC   B8EKC5;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   27-MAR-2024, entry version 83.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN   OrderedLocusNames=Msil_1718 {ECO:0000313|EMBL:ACK50665.1};
OS   Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB
OS   13906 / BL2).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Beijerinckiaceae; Methylocella.
OX   NCBI_TaxID=395965 {ECO:0000313|EMBL:ACK50665.1, ECO:0000313|Proteomes:UP000002257};
RN   [1] {ECO:0000313|EMBL:ACK50665.1, ECO:0000313|Proteomes:UP000002257}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2
RC   {ECO:0000313|Proteomes:UP000002257};
RX   PubMed=20472789; DOI=10.1128/JB.00506-10;
RA   Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B.,
RA   Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.;
RT   "Complete genome sequence of the aerobic facultative methanotroph
RT   Methylocella silvestris BL2.";
RL   J. Bacteriol. 192:3840-3841(2010).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; CP001280; ACK50665.1; -; Genomic_DNA.
DR   AlphaFoldDB; B8EKC5; -.
DR   STRING; 395965.Msil_1718; -.
DR   KEGG; msl:Msil_1718; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_5; -.
DR   OrthoDB; 9768133at2; -.
DR   Proteomes; UP000002257; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF32; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Pyruvate {ECO:0000313|EMBL:ACK50665.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002257}.
FT   ACT_SITE        141
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
FT   ACT_SITE        578
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   919 AA;  102507 MW;  DB7EC507D4F1DB02 CRC64;
     MTELQAERRL EQALSVVDVS DGAPSGERIA AILYQLLLEV VQRHHPEIAP VLSGETVLQN
     AAPEVLSRVF QAHGIWFQLL SIVEQDAAMG ERRRTERELG EQAVPGTFAN VIAKAAKLGV
     PAADIAEKLG RTRVRPVITA HPTEAKRVTV LEKHRRIYRL LVELEQPRWT GRERSGIIDQ
     LRDEIELLWM TGELRLEKPS VEQEIAWGLH FFHETLFEGV PELLKKFDRA LKRFYPSENF
     ALTQFFQFGS WIGGDRDGNP FVTNKVTRRA LRENAKASLR FYEQRLFNLM KSLSISERAL
     PPPSGFREAL NRALSDSGDG AGIALRNPGE PYRQFLYCAH AKVKATLALY EGERTPGPHY
     ISADDLISDL AVTEQALAES GSVGLAQDLV RPVRFAVEAF RFRTQRLDLR ENTTKLTSTL
     QALWRAMHTE ADGEPPAVES DEWKAWILAE LARPRIGPPV YRALPPEAEE TLGMFKLARD
     RDEEFDREAF GSFVLSMTRS AADVLGAYLL AKEADLYHDA TRVEACTLSI TPLFETITDL
     RAAPQIMREL LSLPLVRRSA RMQGGVQEVM IGYSDSNKDG GFLSSNWELA KAQDKLAKIG
     AEAGIPISFF HGRGGSVSRG GAPTAQAIAA QPAGSIQGRL RVTEQGEVVS FKYANRGTAA
     FQMEMLGSSV FEHTLTLERA APALRAEFEE AMEALSGASH AAYANFINHP DLLTYFQSAS
     PLDEISMLNI GSRPARRFGA RTLSDLRAIP WVFAWAQNRH VITGWYGVGS GIASFLDVRK
     ERGLTLLKRM FAESDLFRMI VGEVEKTLGM VDLDIAREYA GLVYDDDVRE TIFGMIEAEY
     RLTRANILKI TGEEEIAQRF PAYRSRLETR LKTINKVNRE QVELLRNFRA STNELSKEAF
     KSNLLLSINC ISSGLGATG
//