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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 738ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSIL395965:GCND-1741-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:Msil_1712
OrganismiMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
ProteomesiUP000002257: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Formate--tetrahydrofolate ligasePRO_1000185259Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi395965.Msil_1712.

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8EKB9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASDIEIAQR ATMERITKVA AEKLGIDDEH LEPYGHFKAK ISLDYVDSLK
60 70 80 90 100
DRPDGKLILV TAISPTPAGE GKTTTTVGLG DALNRIGKKA SICLREPSLG
110 120 130 140 150
PVFGMKGGAA GGGYAQVVPM EDINLHFTGD FSAIALATNL LAAMIDNHIH
160 170 180 190 200
HGNELNIDVR RIAWKRVVDM NDRALRDITI ALGGTANGFP RQDGFDIVVA
210 220 230 240 250
SEVMAIFCLA NSIEDLKARL GAIVIGYTTD QKPVHARDLQ ANGAMAVLLK
260 270 280 290 300
NALKPNLVQT LENNPAFIHG GPFANIAHGC NSVLATKTAL KLSDYVVTEA
310 320 330 340 350
GFGADLGAEK FIDIKCRKSG LRPQAVVIVA TIRALKYHGG VELKELNTEN
360 370 380 390 400
LDALRKGLSN LERHINNIRN HYGLPVVVAI NHFTADTAAE VDLLKKSVAD
410 420 430 440 450
LGAPIVVCRH WAEGGKGAED LARVVVEMID KVPSDFHFVY EDSASLWDKA
460 470 480 490 500
TAVATKLYGA TKVTADAKVR NQIKKLEESG YGNFPICIAK TQYSFSTDAK
510 520 530 540 550
LRGAPTGHDI NIREVRLAAG AEFIVLVCGD VMTMPGLPKI PSATKIDLNE

KGEVVGLF
Length:558
Mass (Da):59,937
Last modified:March 3, 2009 - v1
Checksum:iB46A8350F0EE0257
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA. Translation: ACK50659.1.
RefSeqiWP_012590729.1. NC_011666.1.
YP_002362021.1. NC_011666.1.

Genome annotation databases

EnsemblBacteriaiACK50659; ACK50659; Msil_1712.
GeneIDi7093172.
KEGGimsl:Msil_1712.
PATRICi22598959. VBIMetSil55537_1853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA. Translation: ACK50659.1.
RefSeqiWP_012590729.1. NC_011666.1.
YP_002362021.1. NC_011666.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395965.Msil_1712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK50659; ACK50659; Msil_1712.
GeneIDi7093172.
KEGGimsl:Msil_1712.
PATRICi22598959. VBIMetSil55537_1853.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciMSIL395965:GCND-1741-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
    Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
    J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BL2 / DSM 15510 / NCIMB 13906.

Entry informationi

Entry nameiFTHS_METSB
AccessioniPrimary (citable) accession number: B8EKB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 4, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.