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B8EKB9 (FTHS_METSB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Msil_1712
OrganismMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP]
Taxonomic identifier395965 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella

Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 558558Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000185259

Regions

Nucleotide binding66 – 738ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B8EKB9 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: B46A8350F0EE0257

FASTA55859,937
        10         20         30         40         50         60 
MASDIEIAQR ATMERITKVA AEKLGIDDEH LEPYGHFKAK ISLDYVDSLK DRPDGKLILV 

        70         80         90        100        110        120 
TAISPTPAGE GKTTTTVGLG DALNRIGKKA SICLREPSLG PVFGMKGGAA GGGYAQVVPM 

       130        140        150        160        170        180 
EDINLHFTGD FSAIALATNL LAAMIDNHIH HGNELNIDVR RIAWKRVVDM NDRALRDITI 

       190        200        210        220        230        240 
ALGGTANGFP RQDGFDIVVA SEVMAIFCLA NSIEDLKARL GAIVIGYTTD QKPVHARDLQ 

       250        260        270        280        290        300 
ANGAMAVLLK NALKPNLVQT LENNPAFIHG GPFANIAHGC NSVLATKTAL KLSDYVVTEA 

       310        320        330        340        350        360 
GFGADLGAEK FIDIKCRKSG LRPQAVVIVA TIRALKYHGG VELKELNTEN LDALRKGLSN 

       370        380        390        400        410        420 
LERHINNIRN HYGLPVVVAI NHFTADTAAE VDLLKKSVAD LGAPIVVCRH WAEGGKGAED 

       430        440        450        460        470        480 
LARVVVEMID KVPSDFHFVY EDSASLWDKA TAVATKLYGA TKVTADAKVR NQIKKLEESG 

       490        500        510        520        530        540 
YGNFPICIAK TQYSFSTDAK LRGAPTGHDI NIREVRLAAG AEFIVLVCGD VMTMPGLPKI 

       550 
PSATKIDLNE KGEVVGLF 

« Hide

References

[1]"Complete sequence of Methylocella silvestris BL2."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. expand/collapse author list , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001280 Genomic DNA. Translation: ACK50659.1.
RefSeqYP_002362021.1. NC_011666.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395965.Msil_1712.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK50659; ACK50659; Msil_1712.
GeneID7093172.
KEGGmsl:Msil_1712.
PATRIC22598959. VBIMetSil55537_1853.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycMSIL395965:GCND-1741-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_METSB
AccessionPrimary (citable) accession number: B8EKB9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways