B8EKB9 (FTHS_METSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Msil_1712

Organism

Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906) [Complete proteome] [HAMAP]

Taxonomic identifier

395965 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Methylocella

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_1000185259
Regions
	Nucleotide binding	66 – 73	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8EKB9 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: B46A8350F0EE0257	FASTA	558	59,937
	10 20 30 40 50 60 MASDIEIAQR ATMERITKVA AEKLGIDDEH LEPYGHFKAK ISLDYVDSLK DRPDGKLILV 70 80 90 100 110 120 TAISPTPAGE GKTTTTVGLG DALNRIGKKA SICLREPSLG PVFGMKGGAA GGGYAQVVPM 130 140 150 160 170 180 EDINLHFTGD FSAIALATNL LAAMIDNHIH HGNELNIDVR RIAWKRVVDM NDRALRDITI 190 200 210 220 230 240 ALGGTANGFP RQDGFDIVVA SEVMAIFCLA NSIEDLKARL GAIVIGYTTD QKPVHARDLQ 250 260 270 280 290 300 ANGAMAVLLK NALKPNLVQT LENNPAFIHG GPFANIAHGC NSVLATKTAL KLSDYVVTEA 310 320 330 340 350 360 GFGADLGAEK FIDIKCRKSG LRPQAVVIVA TIRALKYHGG VELKELNTEN LDALRKGLSN 370 380 390 400 410 420 LERHINNIRN HYGLPVVVAI NHFTADTAAE VDLLKKSVAD LGAPIVVCRH WAEGGKGAED 430 440 450 460 470 480 LARVVVEMID KVPSDFHFVY EDSASLWDKA TAVATKLYGA TKVTADAKVR NQIKKLEESG 490 500 510 520 530 540 YGNFPICIAK TQYSFSTDAK LRGAPTGHDI NIREVRLAAG AEFIVLVCGD VMTMPGLPKI 550 PSATKIDLNE KGEVVGLF

References

[1]

"Complete sequence of Methylocella silvestris BL2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Forester B., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Dunfield P.F., Dedysh S.N., Liesack W. , Stott M.B., Smirnova A.V., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BL2 / DSM 15510 / NCIMB 13906.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001280 Genomic DNA. Translation: ACK50659.1.
RefSeq	YP_002362021.1. NC_011666.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B8EKB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7093172.
GenomeReviews	Gene locus Msil_1712 in contig CP001280_GR.
KEGG	msl:Msil_1712.
PATRIC	22598959. VBIMetSil55537_1853.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG677721.
OMA	TDFTVTI.
ProtClustDB	PRK13505.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_METSB

Accession

Primary (citable) accession number: B8EKB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program