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Protein

Ribonuclease 3

Gene

rnc

Organism
Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45MagnesiumUniRule annotation1
Active sitei49UniRule annotation1
Metal bindingi117MagnesiumUniRule annotation1
Active sitei120UniRule annotation1
Metal bindingi120MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, rRNA-binding
Biological processmRNA processing, rRNA processing, tRNA processing
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:Msil_0991
OrganismiMethylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Taxonomic identifieri395965 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
Proteomesi
  • UP000002257 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001189271 – 235Ribonuclease 3Add BLAST235

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi395965.Msil_0991.

Structurei

3D structure databases

SMRiB8EK13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 131RNase IIIUniRule annotationAdd BLAST125
Domaini156 – 225DRBMUniRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the ribonuclease III family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZBM. Bacteria.
COG0571. LUCA.
HOGENOMiHOG000246809.
KOiK03685.
OMAiLTHKSCK.
OrthoDBiPOG091H00R1.

Family and domain databases

CDDicd00593. RIBOc. 1 hit.
Gene3Di1.10.1520.10. 1 hit.
HAMAPiMF_00104. RNase_III. 1 hit.
InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
PfamiView protein in Pfam
PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiView protein in PROSITE
PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.

Sequencei

Sequence statusi: Complete.

B8EK13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAPDLSAL EARIGHRFSA ADLIEAALTH VSAAQGKRVS YQRLEFLGDR
60 70 80 90 100
VLGVVVANML YGAFPDADEG ELSRRLADLV RKESCAEVAL EWGVEAYVRV
110 120 130 140 150
GESEKQNASV NRAILGDVCE SIIGAVFLDG GFAPAQRVVT QAFEPRMRSP
160 170 180 190 200
RRPLRDPKTT LQEWAQARGL PPPSYRETAR TGPDHAPEFT ISVEIAGFQQ
210 220 230
AEARGFAKRL AEQAAAAAFL TREKIVEPGD RKGAA
Length:235
Mass (Da):25,443
Last modified:March 3, 2009 - v1
Checksum:i7C40A9482385901D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA. Translation: ACK49960.1.
RefSeqiWP_012590030.1. NC_011666.1.

Genome annotation databases

EnsemblBacteriaiACK49960; ACK49960; Msil_0991.
KEGGimsl:Msil_0991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001280 Genomic DNA. Translation: ACK49960.1.
RefSeqiWP_012590030.1. NC_011666.1.

3D structure databases

SMRiB8EK13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395965.Msil_0991.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK49960; ACK49960; Msil_0991.
KEGGimsl:Msil_0991.

Phylogenomic databases

eggNOGiENOG4108ZBM. Bacteria.
COG0571. LUCA.
HOGENOMiHOG000246809.
KOiK03685.
OMAiLTHKSCK.
OrthoDBiPOG091H00R1.

Family and domain databases

CDDicd00593. RIBOc. 1 hit.
Gene3Di1.10.1520.10. 1 hit.
HAMAPiMF_00104. RNase_III. 1 hit.
InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
PfamiView protein in Pfam
PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiView protein in PROSITE
PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNC_METSB
AccessioniPrimary (citable) accession number: B8EK13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: June 7, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.