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B8EJI4

- RUVC_METSB

UniProt

B8EJI4 - RUVC_METSB

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Methylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121MagnesiumUniRule annotation
    Metal bindingi72 – 721MagnesiumUniRule annotation
    Metal bindingi144 – 1441MagnesiumUniRule annotation
    Metal bindingi147 – 1471MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMSIL395965:GCND-2-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:Msil_0002
    OrganismiMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
    Taxonomic identifieri395965 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeMethylocella
    ProteomesiUP000002257: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 176176Crossover junction endodeoxyribonuclease RuvCPRO_1000195263Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi395965.Msil_0002.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012181.
    KOiK01159.
    OMAiRQVQEMV.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8EJI4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRAAIRIIG IDPGLRNMGW GVIEASGSRL SFIACGSVHS QAGSSLAERL    50
    CALHEGLLGV IADLSPMEAA VEETFVNCDP QSALKLGQAR GVALVAPALA 100
    GLPVAEYAAN LIKKTVVGNG HAEKAQIAMM VKFLLPMSEA KSADAADALA 150
    VAITHAQLRA SRALLKSVSS MSVQTR 176
    Length:176
    Mass (Da):18,201
    Last modified:March 3, 2009 - v1
    Checksum:iC398EEFB77073D46
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001280 Genomic DNA. Translation: ACK48987.1.
    RefSeqiYP_002360349.1. NC_011666.1.

    Genome annotation databases

    EnsemblBacteriaiACK48987; ACK48987; Msil_0002.
    GeneIDi7092330.
    KEGGimsl:Msil_0002.
    PATRICi22595203. VBIMetSil55537_0002.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001280 Genomic DNA. Translation: ACK48987.1 .
    RefSeqi YP_002360349.1. NC_011666.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395965.Msil_0002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK48987 ; ACK48987 ; Msil_0002 .
    GeneIDi 7092330.
    KEGGi msl:Msil_0002.
    PATRICi 22595203. VBIMetSil55537_0002.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012181.
    KOi K01159.
    OMAi RQVQEMV.
    OrthoDBi EOG6RG044.

    Enzyme and pathway databases

    BioCyci MSIL395965:GCND-2-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2."
      Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B., Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.
      J. Bacteriol. 192:3840-3841(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BL2 / DSM 15510 / NCIMB 13906.

    Entry informationi

    Entry nameiRUVC_METSB
    AccessioniPrimary (citable) accession number: B8EJI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3